Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/346853
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Title

Atypical Mutational Spectrum of SARS-CoV-2 Replicating in the Presence of Ribavirin

AuthorsSomovilla, Pilar; García-Crespo, Carlos; Martínez-González, Brenda; Soria, María Eugenia; Ávila, Ana Isabel de; Gallego, Isabel CSIC ORCID; Mínguez, Pablo; Durán-Pastor, Antoni; Ferrer-Orta, Cristina CSIC ORCID ; Salar-Vidal, Llanos; Esteban-Muñoz, Mario; Zúñiga Lucas, Sonia CSIC ORCID ; Solá Gurpegui, Isabel CSIC ORCID ; Enjuanes Sánchez, Luis CSIC ORCID ; Esteban, Jaime; Fernandez-Roblas, Ricardo; Gadea, Ignacio; Gómez-Castilla, Jordi CSIC ORCID; Verdaguer, Núria CSIC ORCID ; Domingo, Esteban CSIC ORCID; Perales, Celia CSIC ORCID
KeywordsSARS-CoV-2
COVID-19
Viral quasispecies
Ribavirin
Lethal mutagenesis
Issue Date5-Jan-2023
PublisherAmerican Society for Microbiology
CitationAntimicrobial Agents and Chemotherapy 67(1): e01315-22 (2023)
AbstractWe report that ribavirin exerts an inhibitory and mutagenic activity on SARS-CoV-2-infecting Vero cells, with a therapeutic index higher than 10. Deep sequencing analysis of the mutant spectrum of SARS-CoV-2 replicating in the absence or presence of ribavirin indicated an increase in the number of mutations, but not in deletions, and modification of diversity indices, expected from a mutagenic activity. Notably, the major mutation types enhanced by replication in the presence of ribavirin were A→G and U→C transitions, a pattern which is opposite to the dominance of G→A and C→U transitions previously described for most RNA viruses. Implications of the inhibitory activity of ribavirin, and the atypical mutational bias produced on SARS-CoV-2, for the search for synergistic anti-COVID-19 lethal mutagen combinations are discussed.
Publisher version (URL)https://doi.org/10.1128/aac.01315-22
URIhttp://hdl.handle.net/10261/346853
DOI10.1128/aac.01315-22
ISSN0066-4804
E-ISSN1098-6596
Appears in Collections:(CBM) Artículos
(PTI Salud Global) Colección Especial COVID-19
(CNB) Artículos
(IPBLN) Artículos
(IBMB) Artículos




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