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Title

Protective effect of vitamin C against the ethanol mediated toxic effects on human brain glial cells

AuthorsSánchez-Moreno, Concepción CSIC ORCID ; Paniagua, Manuel; Madrid, Antonio; Martín, Antonio CSIC ORCID
KeywordsGlial cells
Ethanol
Cell viability
Vitamin C
Hsp70
COX-2
PGE2
Issue DateOct-2003
PublisherElsevier
CitationJournal of Nutritional Biochemistry 14(10): 606-613 (2003)
AbstractIt is now known that chronic consumption of excessive amounts of alcohol is a major source of social and medical problems. Ethanol-mediated glial cell activation may lead to neuron damage in many ways, including the formation of free radicals and production of pro-inflammatory molecules. Vitamin C (vit-C) is an essential dietary nutrient required as a co-factor for many enzymes and a very efficient antioxidant, protecting cells against free radical-mediated damage. The objective of this study was to evaluate the protective effects of vit-C on glial cell activation and viability against ethanol-mediated toxicity. Human brain astrocyte cells (HA) were exposed to ethanol (0, 50, and 350 mmol/L) for 24 h. We found that glial cells incubated with different concentrations of vit-C increase their vit-C in a dose-dependent manner. HA incubated with 0, 50 or 350 mmol/L of ethanol for up to 24 h showed toxic effects that were proportional to the levels of ethanol in the medium, HA showed increased levels of heat shock protein (Hsp70). However, cells enriched with vit-C before being exposed to ethanol, were better protected against the alcohol-mediated toxicity than non-supplemented cells, and showed significantly lower concentrations of Hsp70. Ethanol also caused increased expression of cyclooxygenase-2 (COX-2) and synthesis of prostaglandin E2 (PGE2), which were reduced by vit-C. In summary, HA supplemented with vit-C were significantly more resistant to the ethanol-mediated toxic effects.
Description8 páginas, 4 figuras, 2 tablas.
Publisher version (URL)http://dx.doi.org/10.1016/j.jnutbio.2003.07.003
URIhttp://hdl.handle.net/10261/34608
DOI10.1016/j.jnutbio.2003.07.003
ISSN0955-2863
Appears in Collections:(IF) Artículos

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