English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/34405
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:

DC FieldValueLanguage
dc.contributor.authorArtigas, Francesc-
dc.contributor.authorGelpí, Emili-
dc.date.accessioned2011-04-07T11:16:21Z-
dc.date.available2011-04-07T11:16:21Z-
dc.date.issued1979-01-01-
dc.identifier.citationAnalytical Biochemistry 92 (1) : 233-242 (1979)es_ES
dc.identifier.issn0003-2697-
dc.identifier.urihttp://hdl.handle.net/10261/34405-
dc.description.abstractA new method for the concurrent extraction and quantification of tryptophan (Trp), tryptamine (T), indole-3-acetic acid (IAA), serotonin (5-HT), and 5-hydroxyindole-3-acetic acid (5-HIAA) in samples of rat brain is presented. Homogenization is carried out in 0.1 HCl containing 1 KCl and 0.2% NaHSO3. After centrifugation at 100,000g, the supernatant is percolated through a column of XAD-2 resin, eluted with distilled methanol, and the resulting eluate is evaporated to dryness. The dry residue is then derivatized to yield the pentafluoropropionated (PFP) and methylpentafluoropropionated (Me-PFP) derivatives. Identification and quantification is readily achieved by gas chromatography-mass fragmentographic analysis on a OV-17 or Dexsil 300 column. Endogenous levels in whole rat brain established by this method are IAA, 13,1 ± 2.0 ng/g (n = 6); T, less than 380 pg/g (n = 6); Trp, 4.16 ± 0.23 μg/g (n = 6); 5-HIAA, 442 ± 24 ng/g (n = 6); and 5-HT, 526 ± 81 ng/g (n = 5).es_ES
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.rightsclosedAccesses_ES
dc.titleA new mass fragmentographic method for the simultaneous analysis of tryptophan, tryptamine, indole-3-acetic acid, serotonin, and 5-hydroxyindole-3-acetic acid in the same sample of rat braines_ES
dc.typeartículoes_ES
dc.identifier.doi10.1016/0003-2697(79)90651-1-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1016/0003-2697(79)90651-1es_ES
dc.identifier.e-issn1096-0309-
Appears in Collections:(IIBB) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
View/Open
Show simple item record
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.