English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/3435
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Citado 33 veces en Web of Knowledge®  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar EndNote (RIS)Exportar EndNote (RIS)
Título : An imperfect heat shock element and different upstream sequences are required for the seed-specific expression of a small heat shock protein gene.
Autor : Carranco, Raúl ; Almoguera, Concepción ; Jordano, Juan
Palabras clave : Heat shock element
Upstream sequences
Seed-specific expression
Heat shock protein gene
Transgenic tobacco
Nicotiana tabacum
Sunflower
Zygotic embryogenesis
Seed desiccation
Mutagenesis
Transgene expression
Factor
Seeds
Gene promoters
Fecha de publicación : nov-1999
Editor: American Society of Plant Biologists
Citación : Plant Physiology 121: 723–730 (1999)
Resumen: Chimeric constructs containing the promoter and upstream sequences of Ha hsp17.6 G1, a small heat shock protein gene, reproduced in transgenic tobacco (Nicotiana tabacum) its unique seed-specific expression patterns previously reported in sunflower. These constructs did not respond to heat shock, but were expressed without exogenous stress during late zygotic embryogenesis coincident with seed desiccation. Site-directed mutagenesis of its distal and imperfect heat shock element strongly impaired in vitro heat shock transcription factor binding and transgene expression in seeds. Deletion analyses of upstream sequences indicated the contribution of additional cis-acting elements with either positive or negative effects on transgene expression. These results show differences in the transcriptional activation through the heat shock element of small heat shock protein gene promoters in seeds compared with the heat shock response. In addition, they suggest that heat shock transcription factors and other distinct trans-acting factors cooperate in the regulation of Ha hsp17.6 G1 during seed desiccation.
Versión del editor: http://dx.doi.org/10.1104/pp.121.3.723
URI : 10261/3435
DOI: 10.1104/pp.121.3.723
ISBN : 1532-2548
Aparece en las colecciones: (IRNAS) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 



NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.