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An imperfect heat shock element and different upstream sequences are required for the seed-specific expression of a small heat shock protein gene.

AuthorsCarranco, Raúl ; Almoguera, Concepción ; Jordano, Juan
KeywordsHeat shock element
Upstream sequences
Seed-specific expression
Heat shock protein gene
Transgenic tobacco
Nicotiana tabacum
Zygotic embryogenesis
Seed desiccation
Transgene expression
Gene promoters
Issue DateNov-1999
PublisherAmerican Society of Plant Biologists
CitationPlant Physiology 121: 723–730 (1999)
AbstractChimeric constructs containing the promoter and upstream sequences of Ha hsp17.6 G1, a small heat shock protein gene, reproduced in transgenic tobacco (Nicotiana tabacum) its unique seed-specific expression patterns previously reported in sunflower. These constructs did not respond to heat shock, but were expressed without exogenous stress during late zygotic embryogenesis coincident with seed desiccation. Site-directed mutagenesis of its distal and imperfect heat shock element strongly impaired in vitro heat shock transcription factor binding and transgene expression in seeds. Deletion analyses of upstream sequences indicated the contribution of additional cis-acting elements with either positive or negative effects on transgene expression. These results show differences in the transcriptional activation through the heat shock element of small heat shock protein gene promoters in seeds compared with the heat shock response. In addition, they suggest that heat shock transcription factors and other distinct trans-acting factors cooperate in the regulation of Ha hsp17.6 G1 during seed desiccation.
Publisher version (URL)http://dx.doi.org/10.1104/pp.121.3.723
Appears in Collections:(IRNAS) Artículos
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