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Título : Validation of a Rapid and Reliable Test for Diagnosis of Chagas' Disease by Detection of Trypanosoma cruzi-Specific Antibodies in Blood of Donors and Patients in Central America
Autor : Ponce, Carlos ; Ponce, Elisa; Vinelli, Elizabeth; Montoya, Alberto; Aguilar, Vilma de; González, Antonio Gustavo; Zingales, Bianca; Rangel-Aldao, Rafael; Levin, Mariano J.; Esfandiari, Javan; Umezawa, Eufrosina S.; Luquetti, Alejandro O.; Silveira, José Franco da
Fecha de publicación : oct-2005
Editor: American Society for Microbiology
Citación : J Clin Microbiol. 2005 October; 43(10): 5065–5068
Resumen: In this study we compared the performance of the Chagas Stat-Pak rapid immunochromatographic test with a standard enzyme-linked immunosorbent assay (ELISA) in the serodiagnosis of Chagas’ disease in Central America. Out of 3,400 blood donor samples, 156 (4.6%) were positive in both assays. Three sera out of 2,084 samples from reference laboratories were negative with the rapid test but positive with the ELISA (99.8% agreement). Agreement of 100% between the two tests was observed with 339 additional sera from patients with cardiopathies and 175 sera from potential blood donors in emergency surgical cases occurring on weekends or at night. In conclusion, Chagas Stat-Pak showed 99.6% and 99.9% sensitivity and specificity, respectively, when assayed with 5,998 serum samples. It is a sensitive and specific alternative to the ELISA, as required in medical emergencies and blood screenings in Central America.
URI : http://hdl.handle.net/10261/3431
DOI: 10.1128/JCM.43.10.5065-5068.2005
ISSN: 1098-660X
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