English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/3418
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Citado 3 veces en Web of Knowledge®  |  Pub MebCentral Ver citas en PubMed Central  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar EndNote (RIS)Exportar EndNote (RIS)
Título : Reversible heat-induced inactivation of chimeric β-glucuronidase in transgenic plants
Autor : Almoguera, Concepción ; Rojas, Anabel ; Jordano, Juan
Palabras clave : Transgenic tobacco
Nicotiana tabacum
Translational fusion
GUS
Transcriptional fusion
sHSP
Sunflower
Helianthus annuus
mRNA
Transgenic plants
Plant proteins
Heat stress
Fecha de publicación : 9-abr-2002
Editor: American Society of Plant Biologists
Citación : Plant Physiology 129(1): 333–341, 2002
Resumen: We compared the expression patterns in transgenic tobacco (Nicotiana tabacum) of two chimeric genes: a translational fusion to ß-glucuronidase (GUS) and a transcriptional fusion, both with the same promoter and 5'-flanking sequences of Ha hsp17.7 G4, a small heat shock protein (sHSP) gene from sunflower (Helianthus annuus). We found that immediately after heat shock, the induced expression from the two fusions in seedlings was similar, considering chimeric mRNA or GUS protein accumulation. Surprisingly, we discovered that the chimeric GUS protein encoded by the translational fusion was mostly inactive in such conditions. We also found that this inactivation was fully reversible. Thus, after returning to control temperature, the GUS activity was fully recovered without substantial changes in GUS protein accumulation. In contrast, we did not find differences in the in vitro heat inactivation of the respective GUS proteins. Insolubilization of the chimeric GUS protein correlated with its inactivation, as indicated by immunoprecipitation analyses. The inclusion in another chimeric gene of the 21 amino-terminal amino acids from a different sHSP lead to a comparable reversible inactivation. That effect not only illustrates unexpected post-translational problems, but may also point to sequences involved in interactions specific to sHSPs and in vivo heat stress conditions.
Versión del editor: http://www.plantphysiol.org/cgi/content/abstract/129/1/333
URI : http://hdl.handle.net/10261/3418
DOI: 10.1104/pp.000992
ISBN : 1532-2548 Online
Aparece en las colecciones: (IRNAS) Artículos
Ficheros en este ítem:
No hay ficheros asociados a este ítem.
Mostrar el registro completo
 



NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.