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Title

Modified reverse transcriptase of hiv-1 group o

Other TitlesRetrotranscriptasa del vih-1 de grupo o modificada
AuthorsMenéndez-Arias, Luis ; Matamoros Grande, Tania ; Álvarez, Mar
Issue Date18-Nov-2010
CitationWO2010130864 A1
Abstract[EN] The present invention relates to the field of biotechnology. Specifically, the invention relates to isolated reverse transcriptases of human immunodeficiency virus type 1 (HIV-l) group 0, with mutations V75I and E478Q, which have high copying fidelity and thennostability, as well as to the use thereof for the reverse transcription, amplification, and sequencing of a template nucIeic acid. The invention also relates to a method for producing said reverse transcriptases.
[ES] La presente invención se encuadra dentro del campo de la biotecnología. Más concretamente, se refiere a retrotranscriptasas aisladas de un virus de la inmunodeficiencia humana de tipo 1 (VIH-I) de grupo O, con las mutaciones V75I y E478Q, que presentan una elevada fidelidad de copia y termoestabilidad, así como a su uso para llevar a cabo la retrotranscripción, amplificación o secuenciación de un ácido nucleico molde. La invención se refiere, además, a un método para obtener dichas retrotranscriptasas.
URIhttp://hdl.handle.net/10261/33955
Appears in Collections:(CBM) Patentes
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