English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/3394
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Citado 79 veces en Web of Knowledge®  |  Pub MebCentral Ver citas en PubMed Central  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar EndNote (RIS)Exportar EndNote (RIS)
Título

Genomic analysis reveals the major driving forces of bacterial life in the rhizosphere

Autor Matilla, Miguel A.; Espinosa-Urgel, Manuel; Rodríguez-Herva, José J.; Ramos, Juan L.; Ramos-González, María Isabel
Fecha de publicación 4-sep-2007
EditorBioMed Central
Citación Genome Biology 2007, 8:R179
Resumen[Background] Mutualistic interactions less well known than those between rhizobia and legumes are commonly found between plants and bacteria, frequently pseudomonads, which colonize roots and adjacent soil areas (the rhizosphere).
[Results] A global analysis of Pseudomonas putida genes expressed during their interaction with maize roots revealed how a bacterial population adjusts its genetic program to this lifestyle. Differentially expressed genes were identified by comparing rhizosphere-colonizing populations with three distinct controls covering a variety of nutrients, growth phases and life styles (planktonic and sessile). Ninety rhizosphere up-regulated (rup) genes, which were induced relative to all three controls, were identified, whereas there was no repressed gene in common between the experiments. Genes involved in amino acid uptake and metabolism of aromatic compounds were preferentially expressed in the rhizosphere, which reflects the availability of particular nutrients in root exudates. The induction of efflux pumps and enzymes for glutathione metabolism indicates that adaptation to adverse conditions and stress (oxidative) response are crucial for bacterial life in this environment. The finding of a GGDEF/EAL domain response regulator among the induced genes suggests a role for the turnover of the secondary messenger c-diGMP in root colonization. Several mutants in rup genes showed reduced fitness in competitive root colonization.
[Conclusion] Our results show the importance of two selective forces of different nature to colonize the rhizosphere: stress adaptation and availability of particular nutrients. We also identify new traits conferring bacterial survival in this niche and open a way to the characterization of specific signalling and regulatory processes governing the plant-Pseudomonas association.
Descripción The electronic version of this article is the complete one and can be found online at http://genomebiology.com/2007/8/9/R179
URI http://hdl.handle.net/10261/3394
DOI10.1186/gb-2007-8-9-r179
ISSN1465-6914
Aparece en las colecciones: (EEZ) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
gb-2007-8-9-r179.pdfPrincipal1,07 MBAdobe PDFVista previa
Visualizar/Abrir
gb-2007-8-9-r179-s1.xlsArchivo adicional 1164 kBMicrosoft ExcelVisualizar/Abrir
gb-2007-8-9-r179-s2.xlsArchivo adicional 219,5 kBMicrosoft ExcelVisualizar/Abrir
gb-2007-8-9-r179-s3.xlsArchivo adicional 362 kBMicrosoft ExcelVisualizar/Abrir
Mostrar el registro completo
 



NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.