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Título : Specific SHP-2 partitioning in raft domains triggers integrin-mediated signaling via Rho activation
Autor : Lacalle, Rosa Ana; Mira, Emilia; Gómez-Moutón, Concepción; Jiménez Baranda, Sonia; Martínez-Alonso, Carlos; Mañes, Santos
Fecha de publicación : abr-2002
Editor: Rockefeller University Press
Citación : The Journal of Cell Biology, volume 157, number 2, april 15, 2002, pp. 277–289
Resumen: Cell signaling does not occur randomly over the cell surface, but is integrated within cholesterol-enriched membrane domains, termed rafts. By targeting SHP-2 to raft domains or to a non-raft plasma membrane fraction, we studied the functional role of rafts in signaling. Serumdepleted, nonattached cells expressing the raft SHP-2 form, but not non-raft SHP-2, display signaling events resembling those observed after fibronectin attachment, such as 1 integrin clustering, 397 Y-FAK phosphorylation, and ERK activation, and also increases Rho-GTP levels. Expression of C the dominant negative N19Rho abrogates raft-SHP-2–induced signaling, suggesting that Rho activation is a downstream event in SHP-2 signaling. Expression of a catalytic inactive SHP-2 mutant abrogates the adhesion-induced feedback inhibition of Rho activity, suggesting that SHP-2 contributes to adhesion-induced suppression of Rho activity. Because raft recruitment of SHP-2 occurs physiologically after cell attachment, these results provide a mechanism by which SHP-2 may influence cell adhesion and migration by spatially regulating Rho activity.
Descripción : Copyright © by The Rockefeller University Press
URI : http://hdl.handle.net/10261/3387
DOI: 10.1083/jcb.200109031
ISSN: 0021-9525
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