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Title

The effect of desiccation on cell shape in the lichen Parmelia Sulcata taylor

AuthorsBrown, D. H.; Rapsch, Sonia; Beckett, A.; Ascaso, Carmen CSIC ORCID
KeywordsLichen
Desiccation
Cell shape
Contraction
LTSEM
Parmelia sulcata
Issue Date1987
PublisherCambridge University Press
CitationNew Phytologist 105: 295-299 (1987)
AbstractComparisons have been made of the shape of algal cells in the lichen Parmelia sulcata which was subjected to controlled desiccation regimes inducing substantial water loss. The spherical appearance of algal cells obtaiiíed by conventional techniques for transmission electrón microscopy (TEM) was shown by low temperature scanning electrón microscopy (LTSEM) to be the consequence of rapid rehydration during fixation. Collapse of the walls of algal cells and fungal hyphae in the medulla and algal layer when desiccated were observed with LTSEM and shown to be reversible on rehydration. Desiccation-induced contraction of cortical fungal protoplasts was detected with LTSEM. Fixation with osmium vapour only before TEM demonstrated a similar contraction of algal protoplasts.
Description5 pages, figures, photos, and tables statistics.
URIhttp://hdl.handle.net/10261/33865
ISSN0028-646x
Appears in Collections:(IRN) Artículos




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