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Title

The sequence selectivity of KSRP explains its flexibility in the recognition of the RNA targets

AuthorsDíaz-Moreno, Irene ; García-Mayoral, M.F. ; Hollingworth, David; Ramos, Andrés
KeywordsKSRP
RNA
Protein
Metabolism
Issue Date6-Aug-2008
PublisherOxford University Press
CitationNucleic Acids Research 36(16): 5290-5296 (2008)
AbstractK-homology (KH) splicing regulator protein (KSRP) is a multi-domain RNA-binding protein that regulates different steps of mRNA metabolism, from mRNA splicing to mRNA decay, interacting with a broad range of RNA sequences. To understand how KSRP recognizes its different RNA targets it is necessary to define the general rules of KSRP–RNA interaction. We describe here a complete scaffold-independent analysis of the RNA-binding potential of the four KH domains of KSRP. The analysis shows that KH3 binds to the RNA with a significantly higher affinity than the other domains and recognizes specifically a G-rich target. It also demonstrates that the other KH domains of KSRP display different sequence preferences explaining the broad range of targets recognized by the protein. Further, KSRP shows a strong negative selectivity for sequences containing several adjacent Cytosines limiting the target choice of KSRP within single-stranded RNA regions. The in-depth analysis of the RNA-binding potential of the KH domains of KSRP provides us with an understanding of the role of low sequence specificity domains in RNA recognition by multi-domain RNA-binding proteins.
Description7 páginas, 4 figuras, 1 tabla
Publisher version (URL)http://dx.doi.org/10.1093/nar/gkn509
URIhttp://hdl.handle.net/10261/33803
DOI10.1093/nar/gkn509
ISSN0305-1048
E-ISSN1362-4962
Appears in Collections:(IBVF) Artículos
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