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The use of oxyquinoline in chromosome analysis. With appendix: The effect of oxyquinoline on protoplasmic viscosity

Other TitlesUtilización de la oxiquinoleína en análisis de cromosomas
AuthorsTjio, Joe Hin ; Levan, Albert; Stålfelt, M. G.
Issue Date1950
PublisherCSIC - Estación Experimental de Aula Dei (EEAD)
CitationAn. Estac. Exp. Aula Dei 2 (1): 21-64 (1950)
Abstract[EN] Vital pretreatments of root tips with 8-oxyquinoline result in improvements of the chromosome pictures in the folloving respects: Metaphase chromosomes are contracted, the spíndle is destroyed and does not interfere with the spreading of the chromosomes at the squashing. The centromeric apparatus exhibits unusually clear conditions, four distinct centromeric bodies being visible in prophasemetaphase chromosomes. The heterocromatin may be clearly studied during various prophase stages. The oxyquinoline technique has been tested in about 40 plant species, both mono- and dicotyledonous plants, and it proved favourable in most materials, although less so in plants with small chromosomes. Some information is given of the radiomimetic action of oxyquinoline. A possible interpretation of the mode of action of oxyquinoline is made in connection with the increase in plasm viscosity induced by this substance in Helodea leaf cells by STÅLFELT (see appendix of the present paper).
Description44 pág., 19 fig.
Publisher version (URL)http://www.eead.csic.es/index.php?id=137
Appears in Collections:(EEAD) Artículos
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