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dc.contributor.authorDíaz-Martín, J.-
dc.contributor.authorPrieto-Dapena, P.-
dc.contributor.authorJordano, Juan-
dc.contributor.authorEspinosa-Vázquez, José Manuel-
dc.contributor.authorAlmoguera, Concepción-
dc.date.accessioned2008-03-28T07:11:08Z-
dc.date.available2008-03-28T07:11:08Z-
dc.date.issued2005-10-21-
dc.identifier.citationPlant Physiology 139:1483-1494 (2005)en_US
dc.identifier.isbn0032-0889-
dc.identifier.urihttp://hdl.handle.net/10261/3347-
dc.description.abstractHahsp17.6G1 is the promoter of a small heat stress protein (sHSP) from sunflower (Helianthus annuus) that is activated during zygotic embryogenesis, but which does not respond to heat stress. We report here the cloning of a transcription factor (TF), sunflower drought-responsive element binding factor 2 (HaDREB2), by one-hybrid interaction with functional cis-elements in Hahsp17.6G1. We have analyzed the functional interaction between HaDREB2 and a second transcription factor, sunflower heat stress factor A9 (HaHSFA9), which was previously assigned to the regulation of Hahsp17.6G1. HaDREB2 and HaHSFA9 synergistically trans-activate the Hahsp17.6G1 promoter in bombarded sunflower embryos. This synergistic interaction is heat stress factor (HSF) specific and requires the binding of both factors to the promoter. The C-terminal region of HaHSFA9 is sufficient for the HSF specificity. Our results represent an example of a functional interaction between members of the Apetala 2 (HaDREB2) and HSF (HaHSFA9) families of transcription factors. We suggest new roles in zygotic embryogenesis for specific members of the AP2 transcription factor family.en_US
dc.format.extent488602 bytes-
dc.format.extent580674 bytes-
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dc.format.mimetypeapplication/pdf-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypeapplication/pdf-
dc.language.isoengen_US
dc.publisherAmerican Society of Plant Biologistsen_US
dc.rightsclosedAccessen_US
dc.subjectActivator Fuctionen_US
dc.subjectShock Proteinsen_US
dc.subjectExpressionen_US
dc.subjectArabidopsisen_US
dc.subjectEmbryogenesisen_US
dc.subjectDomainen_US
dc.subjectIdentificationen_US
dc.subjectToleranceen_US
dc.subjectSunfloweren_US
dc.subjectNerworken_US
dc.titleFunctional interaction between two transcription factors involved in the developmental regulation of a small heat stress protein gene promoteren_US
dc.typeArtículoen_US
dc.identifier.doi10.1104/pp.105.069963.-
dc.description.peerreviewedPeer revieweden_US
dc.relation.publisherversionhttp://www.plantphysiol.org/cgi/reprint/139/3/1483en_US
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