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Title

PAP1 signaling involves MAPK signal transduction

AuthorsFerrés-Masó, Montse ; Iovanna, Juan Lucio; Closa, Daniel ; Folch-Puy, Emma
KeywordsPAP1
MAPK
Transcriptional factors
AR42J
Chromatin immunoprecipitation
RNApol ChIP
Issue Date12-May-2009
PublisherSpringer
CitationCellular and Molecular Life Sciences 66(13): 2195-2204 (2009)
AbstractPancreatitis-associated protein 1 (PAP1) belongs to the Reg family of secretory proteins. Several important biological roles have been attributed to PAP1 but the signaling pathways activated by this protein remain only partially understood. Here, we describe the intracellular pathways triggered by PAP1 in a pancreatic acinar cell line. Taking advantage of the fact that PAP1 induces its own transcription, we performed ChIP assays to analyze the recruitment of transcriptional factors on its promoter. Our results show that PAP1 increased the transactivation activity of pap1 and the binding on its promoter of the nuclear factors C/EBPβ, P-CREB, P-ELK1, EGR1, STAT3, and ETS2, which are downstream targets of MAPK signaling. p44/42, p38, and JNK MAPKs activity increased after PAP1 treatment. In addition, pharmacological inhibition of these kinases markedly inhibited the induction of pap1 mRNA. Taken together, these results indicated that the mechanism of PAP1 action involves the activation of the MAPK superfamily.
DescriptionEl pdf del artículo es la versión post-print.-- et al.
Publisher version (URL)http://dx.doi.org/10.1007/s00018-009-0040-4
URIhttp://hdl.handle.net/10261/33336
DOI10.1007/s00018-009-0040-4
ISSN1420-682X
E-ISSN1420-9071
Appears in Collections:(IIBB) Artículos
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