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Polyphasic characterization of phosphate-solubilizing bacteria isolated from rhizospheric soil of the north-eastern region of Portugal.

AuthorsValverde Portal, Ángel; Igual, José Mariano ; Cervantes, Emilio
KeywordsPhosphate solubilizing bacteria,
Issue Date2007
CitationFirst International Meeting on Microbial Phosphate Solubilization. Developments in Plant and Soil Sciences. Editors E. Velázquez, C. Rodríguez-Barrueco. Volume 102. 2007, p: 273-276
AbstractPhosphate-solubilizing microorganisms are often used as plant growth promoters. In the frame of a project focused on the isolation of endosymbionts and ectorhizospheric bacteria associated with Phaseolus vulgaris plants growing in a mountain soil at the north-east of Portugal, we obtained six bacterial strains with high phosphate-solubilizing capability, as demonstrated by the formation of ‘halos’ when plated in YED medium supplemented with tricalcium-phosphate. These bacterial strains were characterized by a polyphasic approach using both phenotypic (API 20 NE) and molecular assays (RAPD, with M13 primer, TP-RAPD, and 16S rDNA sequencing). TP-RAPD yielded an identical band patterns in the six strains indicating that they belong to the same bacterial species. The 16S rRNA sequence analysis of a group-representative strain (P4-22) revealed a sequence similarity value of 99.27% with Pseudomonas jessenii. Moreover, the RAPD fingerprints of these six strains did not show differences, indicating that they form a highly homogenous group. This high homogeneity could be a consequence of the recurring agronomical practices used in this region from the antiquity that include organic fertilization and monoculture of Phaseolus vulgaris.
DescriptionFirst International Meeting on Microbial Phosphate Solubilization. Salamanca, Spain, 16-19 july 2002. 4 p.
Publisher version (URL)http://www.springerlink.com/content/978-1-4020-4019-1#section=290601&page=3&locus=0
Appears in Collections:(IRNASA) Libros y partes de libros
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