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Methods and kits to generate mirna- and small rna-expressing vectors, and its application to develop lentiviral expression libraries

AuthorsPintor-Toro, José Antonio ; Moreno-Mateos, Miguel A. ; Valle Rosado, Iván
Issue Date22-Jul-2010
CitationWO2010081842 A2
AbstractThe invention relates to novel expression systems for microRNAs (miRNAs) based on the expression ofthe sense and antisense strand from convergent promoters. The invention also provides a method for the direct c10ning of small RNAs present in an RNA preparation by the direct ligation of the small RNAs present within the preparation to vectors. Interestingly, if the vectors contain convergent promoter regions, the vectors isolated according to the method ofthe invention can be used for the direct expression ofthe small RNA without the need ofusing the DNA encoding for the small RNA precursor moleculeÉl PÉl
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