English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/3316
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Citado 176 veces en Web of Knowledge®  |  Pub MebCentral Ver citas en PubMed Central  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar EndNote (RIS)Exportar EndNote (RIS)
Título : Dynamic redistribution of raft domains as an organizing platform for signaling during cell chemotaxis
Autor : Gómez-Moutón, Concepción; Lacalle, Rosa Ana; Mira, Emilia; Jiménez Baranda, Sonia; Barber, Domingo F.; Carrera, Ana C.; Martínez-Alonso, Carlos; Mañes, Santos
Fecha de publicación : mar-2004
Editor: Rockefeller University Press
Citación : The Journal of Cell Biology, Volume 164, Number 5, March 1, 2004, pp. 759–768
Resumen: Spatially restricted activation of signaling molecules governs critical aspects of cell migration; the mechanism by which this is achieved nonetheless remains unknown. Using time-lapse confocal microscopy, we analyzed dynamic redistribution of lipid rafts in chemoattractant-stimulated leukocytes expressing glycosyl phosphatidylinositol– anchored green fluorescent protein (GFP-GPI). Chemoattractants induced persistent GFP-GPI redistribution to the leading edge raft (L raft) and uropod rafts of Jurkat, HL60, and dimethyl sulfoxide–differentiated HL60 cells in a pertussis toxin–sensitive, actin-dependent manner. A trans-S membrane, nonraft GFP protein was distributed homogeneously in moving cells. A GFP-CCR5 chimera, which partitions in L rafts, accumulated at the leading edge, and CCR5 redistribution coincided with recruitment and activation of phosphatidylinositol-3 kinase in L rafts in polarized, moving cells. Membrane cholesterol depletion impeded raft redistribution and asymmetric recruitment of PI3K to the cell side facing the chemoattractant source. This is the first direct evidence that lipid rafts order spatial signaling in moving mammalian cells, by concentrating the gradient sensing machinery at the leading edge.
Descripción : Copyright © by The Rockefeller University Press
URI : http://hdl.handle.net/10261/3316
DOI: 10.1083/jcb.200309101
ISSN: 0021-9525
Aparece en las colecciones: (CNB) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
387.pdf4,14 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 



NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.