English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/3308
Compartir / Impacto:
Add this article to your Mendeley library MendeleyBASE
Citado 19 veces en Web of Knowledge®  |  Pub MebCentral Ver citas en PubMed Central  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar EndNote (RIS)Exportar EndNote (RIS)
Título : Production of highly knotted DNA by means of cosmid circularization inside phage capsids
Autor : Trigueros, Sonia; Roca, Joaquim
Fecha de publicación : 21-dic-2007
Editor: BioMed Central
Citación : BMC Biotechnology 2007, 7:94
Resumen: [Background] The formation of DNA knots is common during biological transactions. Yet, functional implications of knotted DNA are not fully understood. Moreover, potential applications of DNA molecules condensed by means of knotting remain to be explored. A convenient method to produce abundant highly knotted DNA would be highly valuable for these studies.
[Results] We had previously shown that circularization of the 11.2 kb linear DNA of phage P4 inside its viral capsid generates complex knots by the effect of confinement. We demonstrate here that this mechanism is not restricted to the viral genome. We constructed DNA cosmids as small as 5 kb and introduced them inside P4 capsids. Such cosmids were then recovered as a complex mixture of highly knotted DNA circles. Over 250 μg of knotted cosmid were typically obtained from 1 liter of bacterial culture.
[Conclusion] With this biological system, DNA molecules of varying length and sequence can be shaped into very complex and heterogeneous knotted forms. These molecules can be produced in preparative amounts suitable for systematic studies and applications.
Descripción : This article is available from: http://www.biomedcentral.com/1472-6750/7/94
URI : http://hdl.handle.net/10261/3308
DOI: 10.1186/1472-6750-7-94
ISSN: 1472-6750
Aparece en las colecciones: (IBMB) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
1472-6750-7-94.pdf521,67 kBAdobe PDFVista previa
Mostrar el registro completo

NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.