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Título : Transient expression of homologous hairpin RNA interferes with PVY transmission by aphids
Autor : Vargas, Marisol; Martínez-García, Belén; Díaz-Ruíz, José Ramón ; Tenllado, Francisco
Palabras clave : Plant viruses
Potato Virus Y
Transmission by aphids
Hairpin RNAs
RNA interference
RNA silencing
Fecha de publicación : 19-mar-2008
Editor: BioMed Central
Citación : Virology Journal 5:42(2008)
Resumen: Hairpin RNAs have been used to confer resistance to viruses in plants through RNA silencing. However, it has not been demonstrated that RNA silencing was effective against inoculation by aphids of non-persistently transmitted viruses, the major route of plant virus spread in nature. As a proof-of-principle strategy, we made use of Agrobacterium tumefaciens to transiently express a hairpin RNA homologous to Potato virus Y (PVY) in plant tissues. A complete and specific interference with aphid transmission of PVY was achieved by inducers of RNA silencing, as demonstrated by specific siRNAs accumulation in agroinfiltrated tissues. To our knowledge, this is the first report of successful interference with non-persistent transmission of a plant virus using RNA interference.
URI : http://hdl.handle.net/10261/3306
DOI: 10.1186/1743-422X-5-42
ISSN: 1743-422X
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