English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/3306
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Transient expression of homologous hairpin RNA interferes with PVY transmission by aphids

AutorVargas, Marisol; Martínez-García, Belén; Díaz-Ruíz, José Ramón ; Tenllado, Francisco
Palabras clavePlant viruses
Potato Virus Y
Transmission by aphids
Hairpin RNAs
RNA interference
RNA silencing
Fecha de publicación19-mar-2008
EditorBioMed Central
CitaciónVirology Journal 5:42(2008)
ResumenHairpin RNAs have been used to confer resistance to viruses in plants through RNA silencing. However, it has not been demonstrated that RNA silencing was effective against inoculation by aphids of non-persistently transmitted viruses, the major route of plant virus spread in nature. As a proof-of-principle strategy, we made use of Agrobacterium tumefaciens to transiently express a hairpin RNA homologous to Potato virus Y (PVY) in plant tissues. A complete and specific interference with aphid transmission of PVY was achieved by inducers of RNA silencing, as demonstrated by specific siRNAs accumulation in agroinfiltrated tissues. To our knowledge, this is the first report of successful interference with non-persistent transmission of a plant virus using RNA interference.
URIhttp://hdl.handle.net/10261/3306
DOI10.1186/1743-422X-5-42
ISSN1743-422X
Aparece en las colecciones: (CIB) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
BMC_Virology-Journal_2008_Vargas et al.pdf226,2 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.