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Título: | Pollen from different olive tree cultivars contains varying amounts of the major allergen Ole e 1 |
Autor: | Castro López, Antonio Jesús CSIC ORCID; Alché Ramírez, Juan de Dios CSIC ORCID; Cuevas, Julián; Romero Márquez, Pedro J. CSIC; Alché Ramírez, Víctor; Rodríguez García, María I. CSIC ORCID | Palabras clave: | Allergens Ole e 1 Olea europaea L. Olive trees Cultivar Skin prick test |
Fecha de publicación: | jul-2003 | Editor: | S. Karger AG | Citación: | International Archives of Allergy and Immunology 131(3): 164-173 (2003) | Resumen: | [Background]: Commercial olive pollen from uncertain cultivar origin is the common material used for clinical and biological studies. We aimed to assess the putative heterogeneity of olive cultivars with regard to the presence of the major pollen allergen Ole e 1 and to determine whether these differences have clinical relevance. [Methods]: The Ole e 1 content of several cultivars was determined by immunoblotting and ultrastructural immunocytochemistry and compared to that of a commercially available olive pollen extract designed for diagnosis. Reverse transcription-polymerase chain reaction analysis of Ole e 1 transcripts was also performed. Crude protein extracts were used to carry out skin prick tests (SPTs) on 30 allergic patients in order to evaluate the clinical importance of such differences. [Results]: Ole e 1 was present in all cultivars, although significant quantitative differences were detected. Ole e 1 transcripts positively correlated with the amount of the allergen. Significant variations in the average reactivity of allergic patients to SPTs were observed depending on the cultivar considered. [Conclusions]: The presence of the Ole e 1 allergen in all the cultivars suggests that this allergen may play an essential biological role. The expression of the allergen is controlled at the transcriptional level. The significant differences in the Ole e 1 content are likely responsible for the different average reactivity exhibited by patients to the cultivars studied, although the role of other allergens cannot be excluded. Our results suggest that the use of the commercial pollen mixtures currently available may lead to mistakes in allergy diagnosis and to limited success in immunotherapy. Therefore, further standardization is strongly recommended. | Descripción: | 10 páginas, 5 figuras. | Versión del editor: | http://dx.doi.org/10.1159/000071482 | URI: | http://hdl.handle.net/10261/32732 | DOI: | 10.1159/000071482 | ISSN: | 1018-2438 |
Aparece en las colecciones: | (EEZ) Artículos |
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5.1.34 IARCHALLERIMMUN 2003 131 164.pdf | 1,38 MB | Adobe PDF | Visualizar/Abrir |
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