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Title

Expressión of Ole e 1, the major olive pollen allergen during in vitro pollen germination

AuthorsM'rani-Alaoui, Mohamed; Castro López, Antonio Jesús; Alché Ramírez, Juan de Dios; Wang, Wei; Fernández, Mari Carmen; Rodríguez García, María I.
KeywordsOlea europaea L.
Pollen allergen
Ole e l
In-vitro germination
SDS- PAGE
Northern blot
Immunolocalization
Endoplasmic reticulum
Issue Date2002
PublisherInternational Society for Horticultural Science
CitationActa Horticulturae 586: 465-468 (2002)
AbstractThe presence of the protein Ole e 1 (the olive pollen major allergen) and its transcripts during in-vitro pollen germination have been analyzed. The results obtained showed that: a) a large amount of the protein (with three variants) was found in both the mature and the germinating pollen grain, b) the protein was also secreted to the aqueous germination medium, c) Ole e 1 gene was highly expressed in both the mature and the germinating pollen grain, and d) the allergen was immunolocalized in association with dilated rough endoplasmic reticulum cisternae. These findings clearly support the idea of Ole e 1 playing a role of paramount importance in pollen germination and pollen tube growth.
Description4 páginas.-- Trabajo presentado en el IV International Symposium on Olive Growing, Italia, Sep. 2000.
Publisher version (URL)http://www.actahort.org/books/586/586_96.htm
URIhttp://hdl.handle.net/10261/32730
ISSN0567-7572
Appears in Collections:(EEZ) Artículos
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