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Título: | Immunogold probes for light and electron microscopic localization of Ole e I in several Oleaceae pollens |
Autor: | Fernández, Mari Carmen; Olmedilla, Adela CSIC ORCID; Alché Ramírez, Juan de Dios CSIC ORCID; Palomino, Pilar; Lahoz, Carlos; Rodríguez García, María I. CSIC ORCID | Palabras clave: | Ole e I Pollen Oleaceae Immunocytochemistry Silver eilhancement Immunoelectron microscopy Endoplasmic reticulum |
Fecha de publicación: | feb-1996 | Editor: | Histochemical Society | Citación: | Journal of Histochemistry and Cytochemistry 44(2): 151-158 (1996) | Resumen: | We investigated the immunolocalization of the olive major allergen Ole e I and Ole e I-like proteins in pollen from several Oleaceae species [olive (Olea europaea), ash (Fraxinus excelsior), privet (Ligustrum vulgaris), lilac (Syringa vulgare), and forsythia (Forsythia suspensa)]. Crossreactions among different pollens were found in enzyme immunoassays. For immunolocalization with light microscopy we used the silver enhancement technique with three monoclonal antibodies (1D8, 10H1, and 16G2) that recognize three different epitopes of the allergen Ole e I. Our findings show that the silver enhancement technique is very useful when several antibodies are to be used for rapid screening of different materials. MAb 10H1 gave the most precise results and was selected for further immunolocalization studies with transmission electron microscopy. The epitope recognized by this MAb was localized exclusively in the endoplasmic reticulum in olive pollen. In lilac, privet, and ash pollen, most of the reactivity was also seen in the endoplasmic reticulum; however, the 10H1 epitope was not detected in forsythia pollen. | Descripción: | 8 páginas, 13 figuras, 1 tabla. | Versión del editor: | http://dx.doi.org/10.1177/44.2.8609371 | URI: | http://hdl.handle.net/10261/32694 | DOI: | 10.1177/44.2.8609371 | ISSN: | 0022-1554 |
Aparece en las colecciones: | (EEZ) Artículos |
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