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Title

Endoplasmic reticulum as a storage site for allergenic proteins in pollen grains of several Oleaceae

AuthorsRodríguez García, María I.; Fernández, Mari Carmen; Alché Ramírez, Juan de Dios; Olmedilla, Adela
KeywordsAllergenic protein
Oleaceae
Pollen
Endoplasmic reticulum
Immuno-localization
Ultrastructure
Issue Date1995
PublisherSpringer
CitationProtoplasma 187(1-4): 111-116 (1995)
AbstractThe ultrastructure of mature pollen grains of several Oleaceae species (Olea europaea, Fraxinus excelsior, Syringa vulgaris, Ligustrum vulgare, andForsythia suspensa) was studied and the immunolocalization of Ole e I, the major allergen of olige pollen, was determined by immunogold labelling. The five Oleaceae pollens studied here showed different intensities of labelling. The Ole e I allergen was localized throughout the rough endoplasmic reticulum. The absence of gold particles in other cell compartments, such as nuclei, pastids, mitochondria, dictyosomes, lipid bodies, and cell wall, as well as the absence of labelling in control preparations, indicate the specificity of immunolocalization. We conclude that endoplasmic reticulum of the mature pollen grain is a storage site for allergenic proteins and is probably also involved in their synthesis.
Description6 páginas, 2 figuras.-- The original publication is available at www.springerlink.com
Publisher version (URL)http://dx.doi.org/10.1007/BF01280238
URIhttp://hdl.handle.net/10261/32690
DOIhttp://dx.doi.org/10.1007/BF01280238
ISSN0033-183X
Appears in Collections:(EEZ) Artículos
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