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Título: | Ole e I: Epitope Mapping, Cross-Reactivity with Other Oleaceae Pollens and Ultrastructural Localization |
Autor: | Martín-Orozco, Elena; Cárdaba, Blanca; Pozo, Victoria del; Andrés, Belén de; Villalba, Mayte; Gallardo, Soledad; Rodríguez García, María I. CSIC ORCID ; Fernández, Mari Carmen; Alché Ramírez, Juan de Dios CSIC ORCID; Rodríguez, Rosalía; Palomino, Pilar; Lahoz, Carlos | Palabras clave: | Ole e l Olea europaea Epitope mapping Oleaceae pollens, cross-reactivity Tree pollens Ultrastructural localization Olive pollen grain ELISA Immunoblotting Immunocytochemical techniques Monoclonal antibody |
Fecha de publicación: | 1994 | Editor: | S. Karger AG | Citación: | International Archives of Allergy and Immunology 104(2): 160-170 (1994) | Resumen: | Ole e I is the major allergen derived from olive tree pollen (Olea europaea) and it is composed of two polypeptides with molecular weights (MWs) of 18 and 20 kD. A panel of six monoclonal antibodies (mAbs) has been prepared and used to map antigenic determinants on this molecule. Four epitope determinants have been identified on Ole e I. Using the purified mAbs produced against Ole e I, we have analyzed the common epitope determinants in olive (O. europaea) and different Oleaceae pollens: ash (Fraxinus excelsior); privet (Ligustrum vulgare); lilac (Syringa vulgaris), and forsythia (Forsythia suspensa). ELISA showed three reactivity groups depending on the recognition of monoclonal antibodies: (1) olive and ash; (2) olive, ash, privet and lilac; and (3) olive, ash, privet, lilac and forsythia. Immunoblotting studies on Oleaceae pollen extracts with these mAbs showed a very similar cross-reactivity pattern. The 18- and 20-kD MW proteins were present in each pollen, except in the case of forsythia. In this case the reactivity pattern was associated with 50- to 55-kD protein bands. This band was recognized by a pool of sera from olive-allergic patients. Finally, ultrastructural localization of Ole e I antigen was performed on the mature olive pollen grain. Ole e I was located in association with dilated endoplasmic reticulum cisternae. Pollen grain walls, nuclei and cytoplasmic organelles were totally devoid of the allergen. | Descripción: | 11 páginas. | Versión del editor: | http://dx.doi.org/10.1159/000236725 | URI: | http://hdl.handle.net/10261/32682 | DOI: | 10.1159/000236725 | ISSN: | 1018-2438 |
Aparece en las colecciones: | (EEZ) Artículos |
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5.1.10 IARCHALLERIMMUN 1994 104 160.pdf | 2,25 MB | Adobe PDF | Visualizar/Abrir |
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