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Olive cultivar origin is a major cause of polymorphism for Ole e 1 pollen allergen

AutorHamman-Khalifa, Abdel Mounim; Castro López, Antonio Jesús; Jiménez-López, José Carlos; Rodríguez García, María I.; Alché Ramírez, Juan de Dios
Palabras claveOle e 1
Olive cultivars
Olive pollen
Fecha de publicación25-ene-2008
EditorBioMed Central
CitaciónBMC Plant Biology 2008, 8:10
Resumen[Background] Pollens from different olive (Olea euroapea L.) cultivars have been shown to differ significantly in their content in Ole e 1 and in their overall allergenicity. This allergen is, in addition, characterized by a high degree of polymorphism in its sequence. The purpose of this study is to evaluate the putative presence of divergences in Ole e 1 sequences from different olive cultivars.
[Results] RNA from pollen individually collected from 10 olive cultivars was used to amplify Ole e 1 sequences by RT-PCR, and the sequences were analyzed by using different bioinformatics tools. Numerous nucleotide substitutions were detected throughout the sequences, many of which resulted in amino acid substitutions in the deduced protein sequences. In most cases variability within a single variety was much lower than among varieties. Key amino acid changes in comparison with "canonical" sequences previously described in the literature included: a) the substitution of C19 -relevant to the disulphide bond structure of the protein-, b) the presence of an additional N-glycosylation motif, and c) point substitutions affecting regions of Ole e 1 already described like relevant for the immunogenicity/allergenicity of the protein.
[Conclusions] Varietal origin of olive pollen is a major factor determining the diversity of Ole e 1 variants. We consider this information of capital importance for the optimal design of efficient and safe allergen formulations, and useful for the genetic engineering of modified forms of the allergen among other applications.
DescripciónProvisional abstract and full-text PDF file correspond to the article as it appeared upon acceptance. Fully formatted PDF file and abstract versions will be made available soon.-- Paper contains 2 figures and 3 supplementary files.
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olive_cultivar.pdfMain text of the paper1,05 MBAdobe PDFVista previa
supp1.jpgNucleotide sequences alignment1,44 MBJPEGVista previa
supp2.docComparison of nucleotide and deduced amino acid sequences of the different forms of Ole e 1 and Ole e 1-like proteins presented in Figs 1 and 2.358 kBMicrosoft WordVisualizar/Abrir
supp3.docKey amino acid substitutions throughout the Ole e 1 and Ole e 1-like sequences analyzed101 kBMicrosoft WordVisualizar/Abrir
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