English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/3268
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Citado 13 veces en Web of Knowledge®  |  Pub MebCentral Ver citas en PubMed Central  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar EndNote (RIS)Exportar EndNote (RIS)
Título : Olive cultivar origin is a major cause of polymorphism for Ole e 1 pollen allergen
Autor : Hamman-Khalifa, Abdel Mounim; Castro López, Antonio Jesús; Jiménez-López, José Carlos; Rodríguez García, María I.; Alché Ramírez, Juan de Dios
Palabras clave : Ole e 1
Olive cultivars
Olive pollen
Allergenicity
Fecha de publicación : 25-ene-2008
Editor: BioMed Central
Citación : BMC Plant Biology 2008, 8:10
Resumen: [Background] Pollens from different olive (Olea euroapea L.) cultivars have been shown to differ significantly in their content in Ole e 1 and in their overall allergenicity. This allergen is, in addition, characterized by a high degree of polymorphism in its sequence. The purpose of this study is to evaluate the putative presence of divergences in Ole e 1 sequences from different olive cultivars.
[Results] RNA from pollen individually collected from 10 olive cultivars was used to amplify Ole e 1 sequences by RT-PCR, and the sequences were analyzed by using different bioinformatics tools. Numerous nucleotide substitutions were detected throughout the sequences, many of which resulted in amino acid substitutions in the deduced protein sequences. In most cases variability within a single variety was much lower than among varieties. Key amino acid changes in comparison with "canonical" sequences previously described in the literature included: a) the substitution of C19 -relevant to the disulphide bond structure of the protein-, b) the presence of an additional N-glycosylation motif, and c) point substitutions affecting regions of Ole e 1 already described like relevant for the immunogenicity/allergenicity of the protein.
[Conclusions] Varietal origin of olive pollen is a major factor determining the diversity of Ole e 1 variants. We consider this information of capital importance for the optimal design of efficient and safe allergen formulations, and useful for the genetic engineering of modified forms of the allergen among other applications.
Descripción : Provisional abstract and full-text PDF file correspond to the article as it appeared upon acceptance. Fully formatted PDF file and abstract versions will be made available soon.-- Paper contains 2 figures and 3 supplementary files.
URI : http://hdl.handle.net/10261/3268
DOI: 10.1186/1471-2229-8-10
ISSN: 1471-2229
Aparece en las colecciones: (EEZ) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
olive_cultivar.pdfMain text of the paper1,05 MBAdobe PDFVista previa
Visualizar/Abrir
supp1.jpgNucleotide sequences alignment1,44 MBJPEGVista previa
Visualizar/Abrir
supp2.docComparison of nucleotide and deduced amino acid sequences of the different forms of Ole e 1 and Ole e 1-like proteins presented in Figs 1 and 2.358 kBMicrosoft WordVisualizar/Abrir
supp3.docKey amino acid substitutions throughout the Ole e 1 and Ole e 1-like sequences analyzed101 kBMicrosoft WordVisualizar/Abrir
Mostrar el registro completo
 



NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.