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Initial characterization of a potential transcriptional enhancer for the human c-K-ras gene

AuthorsJordano, Juan CSIC ORCID ; Perucho, M.
KeywordsBeta globin
Cell culture
Genetic transcription
Hela cell;
Human cell
Promoter region
Base sequence
DNA-binding proteins
Enhancer elements (genetics)
Issue Date1988
PublisherNature Publishing Group
CitationOncogene 2 (4): 359-366 (1988)
AbstractThe nucleotide sequence of the 5' end distal region of the human c-K-ras gene promoter was determined. This region, coincident with a variable DNAase I hypersensitive site in native chromatin, contains sequence similarities with known enhancers. A 400bp MstII DNA fragment of this region stimulated in cis the correctly initiated transcription of the human β-globin gene in transfected Hela cells. The stimulation of β-globin transcription (5-6 fold) was dependent on the distance and orientation of the c-K-ras sequences and on the presence of the CCAAT and CACCC elements in the β-globin promoter. Interaction of nuclear factors with these c-K-ras sequences was analysed by DNAase I footprinting assays using Hela nuclear extracts. A protein binding to these sequences was identified as nuclear factor 1 (NF-1) by DNAase I competition footprinting experiments. However, disruption of the c-K-ras NF-1 binding site by insertion mutagenesis had no effect on the transcriptional activity of the c-K-ras element.
DescriptionMolecular Sequence Numbers GENBANK, X07536(submitted), Y00666(submitted)
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