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Title: | A method to detect and characterize point mutations in transcribed genes: amplification and overexpression of the mutant cKi-ras allele in human tumor cells |
Authors: | Winter, E.; Yamamoto, Fumiko; Almoguera, Concepción CSIC ORCID ; Perucho, M. | Keywords: | Human tumors Activated ras oncogenes Mutations Condo Pancreatic RNA C-ki-ras genes Tumor cells |
Issue Date: | 1985 | Publisher: | National Academy of Sciences (U.S.) | Citation: | Proceedings of the National Academy of Sciences of the USA Vol. 82, Issue 22, 7575-7579, (1985) | Abstract: | A significant percentage of human tumors contain activated ras oncogenes that have acquired oncogenic potential as a result of somatic point mutations at codon 12 or 61 of the encoded ras gene product. We report here a method to detect and characterize mutations in ras genes that is based on the ability of pancreatic ribonuclease (RNase A; EC 3.1.27.5) to cleave RNA heteroduplexes containing single-base mismatches. Using this method, we show that certain human tumor cells contain mutant c-Ki-ras genes, and we define the nature and position of these mutations. At the same time, we describe the presence and estimate the expression of both normal and mutant c-Ki-ras alleles in the same tumor cells. This method should be useful for the diagnostic detection and characterization of single point mutations in expressed genes. | Publisher version (URL): | http://www.pnas.org/content/82/22/7575.full.pdf | URI: | http://hdl.handle.net/10261/32456 | ISSN: | 0027-8424 | E-ISSN: | 1091-6490 |
Appears in Collections: | (IRNAS) Artículos |
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