English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/32446
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:


Advances in multilabeling for TEM and CLSM using primary antibodies derived from the same species. Application of Fab fragments to co-localize olive pollen allergens

AuthorsMorales, Sonia; Rodríguez García, María I.; Alché Ramírez, Juan de Dios
Fab fragments
Issue DateDec-2010
CitationMicroscopy: Science, Technology, Applications and Education
AbstractThe cell distribution of multiple antigens can be simultaneously revealed by fluorescence and confocal laser scanning microscopy (FM, CLSM) after using antibodies (Abs) conjugated to fluorophores with differential spectral characteristics. The same multiple localization can be carried out at TEM (Transmission Electron Microscopy) by using gold particles of different sizes. However, multilabeling studies often need to use primary Abs derived from the same host species. In these cases, interference between different rounds of Abs must be prevented. Methods available to tackle this need are reviewed, focusing particularly on the use of Fab fragments. Protocols regarding multiple localization of olive pollen targets (Ole e 1, Ole e 2, Ole e 9 and actin) by CLSM and TEM are provided. They relay on the use of primary Abs raised in the same host species (rabbit) and the application of Fab fragments.
DescriptionPages of the mentioned chapter: 607-613 (both included) Publisher: Formatex Research Center
ISBNVolume 1 ISBN (13): 978-84-614-6189-9
Appears in Collections:(EEZ) Libros y partes de libros
Files in This Item:
File Description SizeFormat 
5.2.43 formatex 2010 multilabeling.pdf3,25 MBAdobe PDFThumbnail
Show full item record
Review this work

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.