English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/3242
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Citado 51 veces en Web of Knowledge®  |  Pub MebCentral Ver citas en PubMed Central  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar EndNote (RIS)Exportar EndNote (RIS)
Título : Type I phosphatidylinositol 4-phosphate 5-kinase controls neutrophil polarity and directional movement
Autor : Lacalle, Rosa Ana; Peregil, Rosa M.; Albar, Juan Pablo; Merino Plaza, Ernesto; Martínez-Alonso, Carlos; Mérida, Isabel; Mañes, Santos
Fecha de publicación : dic-2007
Editor: Rockefeller University Press
Citación : The Journal of Cell Biology, Vol. 179, No. 7, pp. 1539-1553
Resumen: Directional cell movement in response to external chemical gradients requires establishment of front–rear asymmetry, which distinguishes an up-gradient protrusive leading edge, where Rac-induced F-actin polymerization takes place, and a down-gradient retractile tail (uropod in leukocytes), where RhoA-mediated actomyosin contraction occurs. The signals that govern this spatial and functional asymmetry are not entirely understood. We show that the human type I phosphatidylinositol 4-phosphate 5-kinase isoform β (PIPKIβ) has a role in organizing signaling at the cell rear. We found that PIPKIβ polarized at the uropod of neutrophil-differentiated HL60 cells. PIPKIβ localization was independent of its lipid kinase activity, but required the 83 C-terminal amino acids, which are not homologous to other PIPKI isoforms. The PIPKIβ C terminus interacted with EBP50 (4.1-ezrin-radixin-moesin (ERM)-binding phosphoprotein 50), which enabled further interactions with ERM proteins and the Rho-GDP dissociation inhibitor (RhoGDI). Knockdown of PIPKIβ with siRNA inhibited cell polarization and impaired cell directionality during dHL60 chemotaxis, suggesting a role for PIPKIβ in these processes.
Descripción : Copyright by The Rockefeller University Press
URI : http://hdl.handle.net/10261/3242
DOI: 10.1083/jcb.200705044
ISBN : 0021-9525
Aparece en las colecciones: (CNB) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
1539.pdf395,25 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 



NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.