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dc.contributor.authorAlmoguera, Concepción-
dc.contributor.authorPrieto-Dapena, P.-
dc.contributor.authorJordano, Juan-
dc.date.accessioned2011-02-04T08:30:10Z-
dc.date.available2011-02-04T08:30:10Z-
dc.date.issued1998-02-
dc.identifier.citationPlant Journal 13( 4): 437-446. (1998)es_ES
dc.identifier.issn0960-7412-
dc.identifier.urihttp://hdl.handle.net/10261/31992-
dc.description.abstractTransgenic tobacco expression was analysed of chimeric genes with point mutations in the heat shock element (HSE) arrays of a small heat shock protein (sHSP) gene from sunflower: Ha hsp17.7 G4. The promoter was developmentally regulated during zygotic embryogenesis and responded to heat stress in vegetative tissues. Mutations in the HSE affected nucleotides crucial for human heat shock transcription factor 1 (HSF1) binding. They abolished the heat shock response of Ha hsp17.7 G4 and produced expression changes that demonstrated dual regulation of this promoter during embryogenesis. Thus, whereas activation of the chimeric genes during early maturation stages did not require intact HSE, expression at later desiccation stages was reduced by mutations in both the proximal (-57 to -89) and distal (-99 to -121) HSE. In contrast, two point mutations in the proximal HSE that did not severely affect gene expression during zygotic embryogenesis, eliminated the heat shock response of the same chimeric gene in vegetative organs. Therefore, by site-directed mutagenesis, it was possible to separate the heat shock response of Ha hsp 17.7 G4 from its developmental regulation. The results indicate the co-existence, in a single promoter, of HSF-dependent and -independent regulation mechanisms that would control sHSP gene expression at different stages during plant embryogenesis.es_ES
dc.description.sponsorshipPPD was supported by a PhD fellowship from the Spanish "Ministerio de Educación y Ciencia". This research was supported by grants BIO96-0474 from Spanish CICYT, and CVI148 from Juanta de Andalucía, awarded to CA and JJ.es_ES
dc.language.isoenges_ES
dc.publisherBlackwell Publishinges_ES
dc.rightsclosedAccesses_ES
dc.subjectGene-expressiones_ES
dc.subjectSeed maturationes_ES
dc.subjectDevelopmental regulationes_ES
dc.subjectProteines_ES
dc.subjectActivationes_ES
dc.subjectLocalizationes_ES
dc.subjectGerminationes_ES
dc.subjectDrosophilaes_ES
dc.subjectTobaccoes_ES
dc.subjectPlantses_ES
dc.titleDual regulation of a heat shock promoter during embryogenesis: stage-dependent role of heat shock elements.es_ES
dc.typeartículoes_ES
dc.identifier.doihttp://dx.doi.org/10.1046/j.1365-313X.1998.00044.x-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1046/j.1365-313X.1998.00044.xes_ES
dc.identifier.e-issn1365-313X-
dc.embargo.terms2015-12-31es_ES
dc.contributor.funderMinisterio de Educación y Ciencia (España)-
dc.contributor.funderComisión Interministerial de Ciencia y Tecnología, CICYT (España)-
dc.contributor.funderJunta de Andalucía-
dc.identifier.funderhttp://dx.doi.org/10.13039/501100007273es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100011011es_ES
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