English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/31992
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:

Title

Dual regulation of a heat shock promoter during embryogenesis: stage-dependent role of heat shock elements.

AuthorsAlmoguera, Concepción CSIC ORCID ; Prieto-Dapena, P. CSIC ORCID; Jordano, Juan CSIC ORCID
KeywordsGene-expression
Seed maturation
Developmental regulation
Protein
Activation
Localization
Germination
Drosophila
Tobacco
Plants
Issue DateFeb-1998
PublisherBlackwell Publishing
CitationPlant Journal 13( 4): 437-446. (1998)
AbstractTransgenic tobacco expression was analysed of chimeric genes with point mutations in the heat shock element (HSE) arrays of a small heat shock protein (sHSP) gene from sunflower: Ha hsp17.7 G4. The promoter was developmentally regulated during zygotic embryogenesis and responded to heat stress in vegetative tissues. Mutations in the HSE affected nucleotides crucial for human heat shock transcription factor 1 (HSF1) binding. They abolished the heat shock response of Ha hsp17.7 G4 and produced expression changes that demonstrated dual regulation of this promoter during embryogenesis. Thus, whereas activation of the chimeric genes during early maturation stages did not require intact HSE, expression at later desiccation stages was reduced by mutations in both the proximal (-57 to -89) and distal (-99 to -121) HSE. In contrast, two point mutations in the proximal HSE that did not severely affect gene expression during zygotic embryogenesis, eliminated the heat shock response of the same chimeric gene in vegetative organs. Therefore, by site-directed mutagenesis, it was possible to separate the heat shock response of Ha hsp 17.7 G4 from its developmental regulation. The results indicate the co-existence, in a single promoter, of HSF-dependent and -independent regulation mechanisms that would control sHSP gene expression at different stages during plant embryogenesis.
Publisher version (URL)http://dx.doi.org/10.1046/j.1365-313X.1998.00044.x
URIhttp://hdl.handle.net/10261/31992
DOIhttp://dx.doi.org/10.1046/j.1365-313X.1998.00044.x
ISSN0960-7412
E-ISSN1365-313X
Appears in Collections:(IRNAS) Artículos
Files in This Item:
There are no files associated with this item.
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.