Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/31948
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Title

Transcriptional activation of a heat shock gene promoter in sunflower embryos: synergism between ABI3 and heat shock factors

AuthorsRojas, Anabel CSIC ORCID; Almoguera, Concepción CSIC ORCID ; Jordano, Juan CSIC ORCID
KeywordsDna-binding activity
Conserver B3 domaim
Seed maturation
C-MYB
Protein
Stress
Elements
Viviparous1
Expression
Tomato
Issue Date5-Jan-2002
PublisherBlackwell Publishing
CitationPlant Journal vol.:20 núm.:5 pág.:601 -610, (1999)
AbstractTransient expression analyses in sunflower embryos demonstrated that ABI3, a seed-specific transcription factor from Arabidopsis, activated chimaeric genes with the Ha hsp17.7 G4 promoter. Nucleotide substitutions at crucial positions of heat shock cis-elements established that they are required for the transcriptional activation involving ABI3. Trans-activation with Lp-HSFA1, a heat shock factor from tomato, reproduced the activation patterns of wild-type and mutant promoters observed with ABI3. In addition, ABI3 and Lp-HSFA1 syner-gistically activated the Ha hsp17.7 G4 promoter, but only when it contained the intact proximal and distal heat shock cis-elements. The activation domain of Lp-HSFA1 was necessary for promoter activation. An amino terminal deletion of ABI3 had dominant negative effects on activation by Lp-HSFA1. We failed to detect a substantial transcriptional activation by ABI3 in the absence of either functional heat shock factors or heat shock elements (HSEs). Furthermore, the wild-type, but not the mutant HSEs (from - 136 to - 49 in Ha hsp17.7 G4) were sufficient, in the context of a - 46 CaMV 35S promoter, to support activation by Lp-HSFA1, or Lp-HSFA1 and ABI3. These results demonstrate, for the first time, transcriptional activation of a heat shock protein promoter by ABI3. We also suggest that ABI3 functions as a transcriptional co-activator through heat shock factors.
Publisher version (URL)http://dx.doi.org/10.1046/j.1365-313X.1999.00635.x
URIhttp://hdl.handle.net/10261/31948
DOI10.1046/j.1365-313X.1999.00635.x
E-ISSN0960-7412
Appears in Collections:(IRNAS) Artículos

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