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Title

Induction of the Hahsp17.7G4 promoter by root-knot nematodes: involvement of heat-shock elements in promoter activity in giant cells

AuthorsEscobar, Carolina; Barcala, Marta; Portillo, Mary; Almoguera, Concepción CSIC ORCID ; Jordano, Juan CSIC ORCID ; Fenoll, Carmen
KeywordsGene-expression
Arabidopsis thaliana
Feeding structures
Developmental regulation
Seed Maturation
Plant
Protein
Tobacco
Embryogenesis
Sequences
Issue DateDec-2003
PublisherAmerican Phytopathological Society
CitationMPMI - Molecular Plant Microbe Interactions vol.16 núm.12 pág.:1062 -1068, (2003)
AbstractRoot-knot nematodes feed from specialized giant cells induced in the plants that they parasitize. We found that the promoter of the Hahsp17.7G4 gene, which encodes a small heat-shock protein involved in embryogenesis and stress responses, directed GUS expression in tobacco galls induced by the root-knot nematode Meloidogyne incognita., In roots containing a GUS reporter fusion to the Hahsp17.7G4 promoter, 10% of the galls stained for GUS expression 1 to 3 days after infection and the fraction stained increased to 60 to 80% 17 to 20 days after infection. A DNA fragment from -83 to +163, which contains heat-shock element (HSE) core sequences, is sufficient to support a promoter activity largely restricted to giant cells within the galls. Two-point mutations in HSE cores, previously reported to abolish the heat-shock response and to strongly reduce the embryogenesis response of the same promoter, did not reduce expression in giant cells. This suggests a distinct regulation of the promoter by nematodes. However, additional point mutations located at positions crucial for binding of heat-shock transcription factors (HSFs) caused a severe decrease in the nematode response. These results demonstrate that HSEs are involved in the promoter activation in giant cells and suggest that HSFs may mediate this response.
Publisher version (URL)http://apsjournals.apsnet.org/doi/pdfplus/10.1094/MPMI.2003.16.12.1062
URIhttp://hdl.handle.net/10261/31911
DOI10.1094/MPMI.2003.16.12.1062
ISBN0894-0282
Appears in Collections:(IRNAS) Artículos

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