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Repression by an auxin/indole acetic acid protein connects auxin signaling with heat shock factormediated seed longevity

AuthorsCarranco, Raúl ; Espinosa-Vázquez, José Manuel; Prieto-Dapena, P. ; Almoguera, Concepción ; Jordano, Juan
Heat shock transcription factor 1
Indoleacetic acid
Down regulation
Nucleotide sequence
Plant seed
Priority journal
Protein interaction
Signal transduction
Transcription initiation
Transgenic plant
Issue Date29-Nov-2010
PublisherNational Academy of Sciences (U.S.)
CitationProceedings of the National Academy of Sciences of the USA Volume 107, Issue 50, 2010, Pages 21908-21913
AbstractThe plant hormone auxin regulates growth and development by modulating the stability of auxin/indole acetic acid (Aux/IAA) proteins, which in turn repress auxin response factors (ARFs) transcriptional regulators. In transient assays performed in immature sunflowerembryos,weobserved that the Aux/IAAproteinHaIAA27 represses transcriptional activation by HaHSFA9, a heat shock transcription factor (HSF). We also found that HaIAA27 is stabilized in immature sunflower embryos, where we could show bimolecular fluorescence complementation interaction between native forms of HaIAA27 and HaHSFA9. An auxin-resistant form of HaIAA27 was overexpressed in transgenic tobacco seeds, leading to effects consistent with down-regulation of the ortholog HSFA9 gene, effects not seen with the native HaIAA27 form. Repression of HSFs by HaIAA27 is thus likely alleviated by auxin in maturing seeds. We show that HSFs such as HaHSFA9 are targets of Aux/IAA protein repression. Because HaHSFA9 controls a genetic program involved in seed longevity and embryonic desiccation tolerance, our findings would suggest a mechanism by which these processes can be auxin regulated. Aux/IAA-mediated repression involves transcription factors distinct from ARFs. This finding widens interpretation of auxin responses.
Description- Molecular Sequence Numbers GENBANK, FR669188(referenced) - Chemicals and CAS Registry Numbers indoleacetic acid, 32536-43-9, 87-51-4
Publisher version (URL)http://www.pnas.org/content/107/50/21908.full.pdf+html
Appears in Collections:(IRNAS) Artículos
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