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Label-free detection of DNA mutations by SPR: application to the early detection of inherited breast cancer

AuthorsCarrascosa, Laura G. ; Calle Martín, Ana ; Lechuga, Laura M.
KeywordsSPR biosensor
DNA hybridization
DNA mutation
Breast cancer
Issue Date2009
CitationAnalytical and Bioanalytical Chemistry 393(4): 1173-1182 (2009)
AbstractA screening analysis of DNA hybridization and the presence of DNA mutations using an surface plasmon resonance (SPR) biosensor is shown. The influence of lateral and vertical spacers, as well as several hybridization conditions, was studied to optimize the differentiation between fully complementary and mismatched DNA strands. Our results demonstrated that SPR biosensors were able to detect mismatch sequences related to inherited breast cancer, with high specificity and sensitivity. Using PCR synthetic sequences as targets, mutant sequences were clearly discriminated from fully complementary ones, and detection limits below 50 nM were achieved.
Description10 páginas, 6 figuras, 2 tablas.
Publisher version (URL)http://dx.doi.org/10.1007/s00216-008-2555-1
Appears in Collections:(CIN2) Artículos
(IMN-CNM) Artículos
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