English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/30894
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

The Rate and Spectrum of Spontaneous Mutations in a Plant RNA Virus

AuthorsTromas, Nicolas; Elena, Santiago F.
KeywordsTasa de mutación
Virus de RNA
Virus de plantas
Carga mutacional
Espectro de mutantes
Evolución experimental
Issue Date2010
PublisherGenetics Society of America
CitationGenetics 185: 983-989 (2010)
AbstractKnowing mutation rates and the molecular spectrum of spontaneous mutations is important to understanding how the genetic composition of viral populations evolves. Previous studies have shown that the rate of spontaneous mutations for RNA viruses widely varies between 0.01 and 2 mutations per genome and generation, with plant RNA viruses always occupying the lower side of this range. However, this peculiarity of plant RNA viruses is based on a very limited number of studies. Here we analyze the spontaneous mutational spectrum and the mutation rate of Tobacco etch potyvirus, a model system of positive sense RNA viruses. Our experimental setup minimizes the action of purifying selection on the mutational spectrum, thus giving a picture of what types of mutations are produced by the viral replicase. As expected for a neutral target, we found that transitions and nonsynonymous (including a few stop codons and small deletions) mutations were the most abundant type. This spectrum was notably different from the one previously described for another plant virus.We have estimated that the spontaneous mutation rate for this virus was in the range 10 6 10 5 mutations per site and generation. Our estimates are in the same biological ballpark that previous values reported for plant RNA viruses. This finding gives further support to the idea that plant RNA viruses may have lower mutation rates than their animal counterparts.
Description7 pags.
DOIDOI: 10.1534/genetics.110.115915
Appears in Collections:(IBMCP) Artículos
Files in This Item:
File Description SizeFormat 
Genetics.pdf528,05 kBAdobe PDFThumbnail
Show full item record
Review this work

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.