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Título

Proteomic analysis of human omental adipose tissue in the polycystic ovary syndrome using two-dimensional difference gel electrophoresis and mass spectrometry

AutorCortón, Marta; Botella-Carretero, José I.; López, Juan A.; Camafeita, Emilio; San Millán, José L.; Escobar-Morreale, Héctor F.; Peral, Belén CSIC ORCID
Palabras claveOxidative stress
Insulin-resistance
Glut4 translocation
Phosphatidylinositol 3-kinase
Cardiovascular diseases
3T3-L1 adipocytes
Androgen excess
Risk-factors
Women
Gene
Fecha de publicaciónmar-2007
EditorOxford University Press
CitaciónHuman Reproduction
ResumenBACKGROUND: Our aim was to study the protein expression profiles of omental adipose tissue biopsies obtained from morbidly obese women with or without polycystic ovary syndrome (PCOS) at the time of bariatric surgery to evaluate the possible involvement of visceral adiposity in the development of PCOS. METHODS: Ten PCOS patients and nine control samples were included. We used two-dimensional difference gel electrophoresis (2D-DIGE) followed by in-gel digestion, and mass spectrometry (MS) of selected protein spots. RESULTS: The 2D-DIGE technology allowed the analysis of similar to 1840 protein spots in the comparative study of control and patient proteomes, revealing 15 statistically significant spot changes (> 2-fold, P < 0.05). Unambiguous protein identification was achieved for 9 of these 15 spots by MS. This preliminary study revealed differences in expression of proteins that may be involved in lipid and glucose metabolism, oxidative stress processes and adipocyte differentiation; they include proapolipoprotein Apo-A1, annexin V, glutathione S-transferase M3 (GSTM3), triosephosphate isomerase, peroxiredoxin 2 isoform a, actin and adipocyte plasma membrane-associated protein. The most relevant finding was an increase of GSTM3 in the omental fat of PCOS patients confirming previous studies conducted by our group. CONCLUSIONS: Proteomic analysis of omental fat reveals differential expression of several proteins in PCOS patients and non-hyperandrogenic women presenting with morbid obesity. The application of this novel methodology adds further evidence to support the role of visceral adiposity in the pathogenesis of PCOS.
Versión del editorhttp://dx.doi.org/10.1093/humrep/dem380
URIhttp://hdl.handle.net/10261/30670
DOI10.1093/humrep/dem380
ISSN0268-1161
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