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dc.contributor.authorPérez Torrado, Robertoen_US
dc.contributor.authorGimeno Alcañiz, José Vicenteen_US
dc.contributor.authorMatallana, Emiliaen_US
dc.identifier.citationApplied and Environmental Microbiology 68(7) : 3339-3344 (2002)en_US
dc.description.abstractWe used metabolic engineering to produce wine yeasts with enhanced resistance to glucose deprivation conditions. Glycogen metabolism was genetically modified to overproduce glycogen by increasing the glycogen synthase activity and eliminating glycogen phosphorylase activity. All of the modified strains had a higher glycogen content at the stationary phase, but accumulation was still regulated during growth. Strains lacking GPH1, which encodes glycogen phosphorylase, are unable to mobilize glycogen. Enhanced viability under glucose deprivation conditions occurs when glycogen accumulates in the strain that overexpresses GSY2, which encodes glycogen synthase and maintains normal glycogen phosphorylase activity. This enhanced viability is observed under laboratory growth conditions and under vinification conditions in synthetic and natural musts. Wines obtained from this modified strain and from the parental wild-type strain don't differ significantly in the analyzed enological parameters. The engineered strain might better resist some stages of nutrient depletion during industrial use.en_US
dc.description.sponsorshipgrant ALI98-1041 from the Comisión Interministerial de Ciencia y Tecnología. GV99-105-1-13 from Generalitat Valenciana. ALI99-1224-CO2-02 from the Comisión Interministerial de Ciencia y Tecnologíaen_US
dc.format.extent315820 bytes-
dc.format.extent2459 bytes-
dc.publisherAmerican Society for Microbiologyen_US
dc.subjectwine yeastsen_US
dc.subjectglycogen phosphorylaseen_US
dc.subjectglucose deprivationen_US
dc.titleWine Yeast Strains Engineered for Glycogen Overproduction Display Enhanced Viability under Glucose Deprivation Conditionsen_US
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