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Title

Lipoxygenase (LOX) in sweet and hot pepper (Capsicum annuum L.) fruits during ripening and under an rnriched nitric oxide (NO) gas atmosphere

AuthorsGonzález-Gordo, Salvador CSIC ORCID; Cañas Rodríguez, Amanda CSIC; Muñoz-Vargas, María A. CSIC ORCID; Palma Martínez, José Manuel CSIC ORCID; Corpas, Francisco J. CSIC ORCID
KeywordsCapsaicin
Isoenzymes
Genes
Lipoxygenase
Nitric oxide
Pepper
Ripening
Issue Date2022
PublisherMolecular Diversity Preservation International
CitationInternational Journal of Molecular Sciences 23: 15211 (2022)
AbstractLipoxygenases (LOXs) catalyze the insertion of molecular oxygen into polyunsaturated fatty acids (PUFA) such as linoleic and linolenic acids, being the first step in the biosynthesis of a large group of biologically active fatty acid (FA)-derived metabolites collectively named oxylipins. LOXs are involved in multiple functions such as the biosynthesis of jasmonic acid (JA) and volatile molecules related to the aroma and flavor production of plant tissues, among others. Using sweet pepper (Capsicum annuum L.) plants as a model, LOX activity was assayed by non-denaturing polyacrylamide gel electrophoresis (PAGE) and specific in-gel activity staining. Thus, we identified a total of seven LOX isozymes (I to VII) distributed among the main plant organs (roots, stems, leaves, and fruits). Furthermore, we studied the FA profile and the LOX isozyme pattern in pepper fruits including a sweet variety (Melchor) and three autochthonous Spanish varieties that have different pungency levels (Piquillo, Padrón, and Alegría riojana). It was observed that the number of LOX isozymes increased as the capsaicin content increased in the fruits. On the other hand, a total of eight CaLOX genes were identified in sweet pepper fruits, and their expression was differentially regulated during ripening and by the treatment with nitric oxide (NO) gas. Finally, a deeper analysis of the LOX IV isoenzyme activity in the presence of nitrosocysteine (CysNO, a NO donor) suggests a regulatory mechanism via S-nitrosation. In summary, our data indicate that the different LOX isozymes are differentially regulated by the capsaicin content, fruit ripening, and NO.
Publisher version (URL)http://dx.doi.org/10.3390/ijms232315211
URIhttp://hdl.handle.net/10261/305250
DOI10.3390/ijms232315211
Identifiersdoi: 10.3390/ijms232315211
issn: 1422-0067
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