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dc.contributor.authorNissen, Lorenzoen_US
dc.contributor.authorYebra, María Jesúsen_US
dc.contributor.authorSgobarti, Barbaraen_US
dc.contributor.authorPérez Martínez, Gasparen_US
dc.date.accessioned2008-02-25T10:36:31Z-
dc.date.available2008-02-25T10:36:31Z-
dc.date.issued2005-04en_US
dc.identifier.citationBiofutura 2005, Bologna, 8-10 Aprile 2005en_US
dc.identifier.urihttp://hdl.handle.net/10261/3051-
dc.description.abstractSorbitol is a sugar polyol claimed to have health-promoting properties. D-sorbitol-6-phosphate dehydrogeanse (StolPDh) is required for sorbose and sorbitol metabolism in Lactobacillus casei. StolPDh catalyzes the oxidation of Sorbitol-6-phosphate and also the reverse reaction, or reather, the reduction of fructose-6-phosphate with NAD+ regeneration. In order to test this function in vivo, a new food grade recombinant strain of L. casei was constructed by the integration of a StolPDh-encoding gene (gutF) in the chromosomal lactose operon. Lactose induction of gutF expression was achieved and sorbitol production could be detected by 13C-nuclear magnetic resonance analys, upon addiction of glucose to resting cells. Additional inactivationn of L-lactate dehydrogenase led to higher sorbitol production. These outcomes indicates that the accumulation of the glycolytic precursor, fructose-6-phosphate and an high NAD+/NADH ratio were the limiting factors for sorbitol accumulation.en_US
dc.format.extent213952 bytes-
dc.format.extent2459 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypetext/plain-
dc.language.isoengen_US
dc.publisherAssociazione Nazionale Biotecnologi Italianien_US
dc.rightsopenAccess-
dc.subjectLactobacillus caseien_US
dc.subjectSorbitolen_US
dc.titleEngineering sorbitol-6-phosphate Dehydrogenase encoding gene in the lactose operon of Lactobacillus casei.en_US
dc.typePresentaciónen_US
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