English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/29754
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:

Title

Bisanthracycline WP631 inhibits basal and Sp1-activated transcription initiation in vitro

AuthorsMartín, Beatriz; Vaquero, Alejandro; Priebe, Waldemar; Portugal, José CSIC ORCID
Issue Date1-Sep-1999
PublisherOxford University Press
CitationNucleic Acids Research 27(17): 3402-3409 (1999)
AbstractAn in vitro transcription assay was used to compare the capacity of the bisintercalating anthracycline WP631 (which displays a remarkably high DNA-binding affinity) and the monointercalating anthracycline daunomycin to inhibit transcription initiation of the adenovirus major late promoter linked to a G-less transcribed DNA template. Both drugs inhibit basal RNA synthesis in a concentration-dependent way, and the drug concentrations required to inhibit transcription initiation are similar. However, in this study WP631 was around 15 times more efficient at inhibiting transcription initiation when used with an adenovirus promoter containing an upstream Sp1-protein binding site under experimental conditions in which the Sp1 protein acted as a transactivator in vitro. The differences in the ability of each drug to inhibit transcription initiation were related to the competition between Sp1 and the drugs for the same binding site. Concentrations of WP631 as low as 60 nM could inhibit the Sp1-activated transcription initiation in vitro. In contrast, the concentration of daunomycin required to inhibit Sp1-activated transcription by 50% was almost the same as the concentration required to inhibit basal transcription. The efficiency of WP631 at displacing Sp1 from its putative binding site was confirmed using gel retardation and footprinting assays. These results are the first unequivocal example of a direct effect of an intercalator on activated transcription initiation.
Description8 pages, 8 figures, 1 table.-- PMID: 10446226 [PubMed].-- PMCID: PMC148580.
Publisher version (URL)http://www.ncbi.nlm.nih.gov/pmc/articles/PMC148580
http://dx.doi.org/10.1093/nar/27.17.3402
URIhttp://hdl.handle.net/10261/29754
DOIhttp://dx.doi.org/10.1093/nar/27.17.3402
ISSN0305-1048
E-ISSN1362-4962
Appears in Collections:(IQAC) Artículos
(IBMB) Artículos
Files in This Item:
File Description SizeFormat 
Martin_Beatriz_et_al.pdf578,85 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.