English
español
Please use this identifier to cite or link to this item:
http://hdl.handle.net/10261/2972
Share/Impact:
Statistics |
![]() ![]() ![]() |
|
|
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |||
|
Title: | Fructose-1,6-biphosphate in rat intestinal preconditioning: involvement of nitric oxide |
Authors: | Solà, Anna M. ![]() ![]() ![]() ![]() |
Keywords: | Fructose-1,6-biphosphate Glyceraldehyde- 3-phosphate dehydrogenase Intestinal preconditioning Ischaemia/reperfusion injury Nitric Oxide |
Issue Date: | Feb-2001 |
Publisher: | BMJ Publishing Group |
Citation: | Gut. 2001 February; 48(2): 168–175. http://dx.doi.org/10.1136/gut.48.2.168 |
Abstract: | [BACKGROUND AND AIMS] Inhibition of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) by nitric oxide (NO) in intestinal preconditioning could modify the rate of formation of glycolytic intermediates. Fructose-1,6-biphosphate (F16BP) is a glycolytic intermediate that protects tissue from ischaemia/reperfusion injury. We evaluated if F16BP may be endogenously accumulated as a consequence of GAPDH inhibition by NO during intestinal preconditioning in rats. [METHODS] We assessed: (1) effect of preconditioning on F16BP content; (2) effect of NO on GAPDH activity before and during sustained ischaemia; and (3) protective effect of F16BP in control, ischaemic, and preconditioned animals with or without administration of N-nitro-L-arginine methyl ester (L-NAME), NO donor, or F16BP. [RESULTS] Preconditioned rats showed a significant transient decrease in GAPDH activity and also maintained basal F16BP levels longer than ischaemic rats. L-NAME administration to preconditioned rats reversed these effects. F16BP administration to ischaemic rats decreased protein release in the perfusate. Administration of F16BP to L-NAME treated rats attenuated the harmful effect of L-NAME. [CONCLUSIONS] Our study indicates that F16BP may be endogenously accumulated in preconditioned rats as a consequence of inhibition of GAPDH by NO, and this may contribute to the protection observed in intestinal preconditioning. |
Publisher version (URL): | http://dx.doi.org/10.1136/gut.48.2.168 |
URI: | http://hdl.handle.net/10261/2972 |
DOI: | http://dx.doi.org/10.1136/gut.48.2.168 |
ISSN: | 0017-5749 |
Appears in Collections: | (IIBB) Artículos |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
fructose.pdf | Text file | 151,04 kB | Adobe PDF | ![]() View/Open |
fructose_f1.jpg | Figure 1.- Intestinal fructose-1,6-biphosphate (F16BP) levels during the different ischaemic periods (0, 2, 30, or 90 minutes) in the control. | 73,74 kB | JPEG | ![]() View/Open |
fructose_f2.jpg | Figure 2.- Intestinal glucose levels during the different ischaemic periods (0, 2, 30, or 90 minutes) in the control. | 47,05 kB | JPEG | ![]() View/Open |
fructose_f3.jpg | Figure 3.- GAPDH activity (U/mg protein) in the intestine during the different ischaemic periods (0, 2, 30, or 90 minutes) in the control. | 73,04 kB | JPEG | ![]() View/Open |
fructose_f4.jpg | Figure 4.- Nitrate and nitrite tissue production in the intestine in the following groups: control, control with previous administration of L-NAME (C+NAME), preconditioning. | 45,75 kB | JPEG | ![]() View/Open |
fructose_f5.jpg | Figure 5.- Profiles of protein release during the different ischaemic (0, 2, 30, and 90 minutes) and reperfusion. | 53,72 kB | JPEG | ![]() View/Open |
fructose_f6.jpg | Figure 6.- Intestinal fructose-1,6-biphosphate (F16BP) levels incorporated in the intestine. | 19,54 kB | JPEG | ![]() View/Open |
Show full item record
Review this work
Review this work
WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.