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Title

Immunolocalization of alkaloids and X-ray microanalysis of elements in lupin seeds

AuthorsPozuelo, José Manuel; Lucas, M. Mercedes ; Lorenzo, C. de; Fernández-Pascual, Mercedes ; Maldonado, Susana; Felipe, Mª Rosario de
KeywordsAlkaloids
Lupinus spp
Immunocytochemistry
X-ray microanalysis
Protein bodies
Issue Date2001
PublisherSpringer
CitationProtoplasma 218:104-111 (2001)
AbstractImmunolocalization of alkaloids in lupin seeds (Lupinus spp.) has been performed by cryofixation and conventional methods. Alkaloids were localized in the protein bodies of the cotyledon ceils. Some immunogold particles in the walls of these cells were also observed. There were no differences in the sites of localization between the two mentioned methods. X-ray microanalysis of elements showed the presence of R Mg, S, and K in the protein bodies of cotyledon cells in lupin seeds. The role of K + in alkaloids transport is discussed.
Description8 pages, figures, and tables statistics.
URIhttp://hdl.handle.net/10261/29696
ISSN0033-183x
Appears in Collections:(ICA) Artículos
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