English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/2917
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Changes in photosynthetic electron transfer and state transitions in an herbicide-resistant D1 mutant from soybean cell cultures

AutorRoncel Gil, Mercedes ; Yruela Guerrero, Inmaculada ; Kirilovsky, D.; Guerrero, Fernando ; Alfonso Lozano, Miguel ; Picorel Castaño, Rafael ; Ortega, José M.
Palabras claveCyclic and linear electron flow
Photosystem I
Photosystem II
Thermoluminiscence
State transitions
D1 mutant
Fecha de publicaciónjun-2007
EditorElsevier
CitaciónBiochimica et Biophysica Acta (BBA) - Bioenergetics 1767 (2007) 694-702.
ResumenAnomalies in photosynthetic activity of the soybean cell line STR7, carrying a single mutation (S268P) in the chloroplastic gene psbA that codes for the D1 protein of the photosystem II, have been examined using different spectroscopic techniques. Thermoluminescence emission experiments have shown important differences between STR7 mutant and wild type cells. The afterglow band induced by both white light flashes and far-red continuous illumination was downshifted by about 4 °C and the Q band was upshifted by 5 °C. High temperature thermoluminescence measurements suggested a higher level of lipid peroxidation in mutant thylakoid membranes. In addition, the reduction rate of P700+ was significantly accelerated in STR7 suggesting that the mutation led to an activation of the photosystem I cyclic electron flow. Modulated fluorescence measurements performed at room temperature as well as fluorescence emission spectra at 77 K revealed that the STR7 mutant is defective in state transitions. Here, we discuss the hypothesis that activation of the cyclic electron flow in STR7 cells may be a mechanism to compensate the reduced activity of photosystem II caused by the mutation. We also propose that the impaired state transitions in the STR7 cells may be due to alterations in thylakoid membrane properties induced by a low content of unsaturated lipids.
DescripciónThe definitive version is available at: http://www.sciencedirect.com/science//journal/00052728
URIhttp://hdl.handle.net/10261/2917
DOI10.1016/j.bbabio.2007.02.017
ISSN0005-2728
Aparece en las colecciones: (IBVF) Artículos
(EEAD) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
BBA17617(2007)694-702 revised.pdf313,88 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.