English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/2911
Compartir / Impacto:
Add this article to your Mendeley library MendeleyBASE
Citado 14 veces en Web of Knowledge®  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar EndNote (RIS)Exportar EndNote (RIS)
Título : Excess copper induces structural changes in cultured photosynthetic soybean cells
Autor : Bernal Ibáñez, María ; Testillano, P.S. ; Risueño, María Carmen ; Yruela Guerrero, Inmaculada
Palabras clave : Acclimation
Microscopic structural analysis
Organic acids
Soybean cell culture
Fecha de publicación : 1-nov-2006
Editor: Commonwealth Scientific and Industrial Research Organization (Australia)
Citación : Functional Plant Biology 33(11): 1001–1012 (2006)
Resumen: Soybean [Glycine max (L.) Merr.] cell suspensions have the capacity to develop tolerance to excess copper, constituting a convenient system for studies on the mechanisms of copper tolerance. The functional cell organisation changes observed in these cell cultures after both short-term (stressed cells) and long-term (acclimated cells) exposure to 10 μm CuSO4 are reported from structural, cytochemical and microanalytical approaches. Cells grown in the presence of 10 μm CuSO4 shared some structural features with untreated cells, such as: (i) a large cytoplasmic vacuole, (ii) chloroplasts along the thin layer of cytoplasm, (iii) nucleus in a peripheral location exhibiting circular-shaped nucleolus and a decondensed chromatin pattern, and (iv) presence of Cajal bodies in the cell nuclei. In addition, cells exposed to 10 μm CuSO4 exhibited important differences compared with untreated cells: (i) chloroplasts displayed rounded shape and smaller size with denser-structured internal membranes, especially in copper-acclimated cells; (ii) no starch granules were found within chloroplasts; (iii) the cytoplasmic vacuole was larger, especially after long-term copper exposure; (iv) the levels of citrate and malate increased. Extracellular dark-coloured deposits with high copper content attached at the outer surface of the cell wall were observed only in cells exposed to a short-term copper stress. Structural cell modifications, mainly affecting chloroplasts, accompanied the short-term copper-induced response and were maintained as stable characters during the period of adaptation to excess copper. Vacuolar changes accompanied the long-term copper response. The results indicate that the first response of soybean cells to excess copper prevents its entry into the cell by immobilising it in the cell wall, and after an adaptive period, acclimation to excess copper may be mainly due to vacuolar sequestration.
Versión del editor: http://dx.doi.org/10.1071/FP06174
URI : http://hdl.handle.net/10261/2911
DOI: 10.1071/FP06174
ISSN: 1445-4408
Aparece en las colecciones: (EEAD) Artículos
(CIB) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
FP06174-R1. (Text and Tables and Figures). doc.pdf1,56 MBAdobe PDFVista previa
Mostrar el registro completo

NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.