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dc.contributor.authorBernal Ibáñez, María-
dc.contributor.authorRamiro Pascual, María Victoria-
dc.contributor.authorCases, Rafael-
dc.contributor.authorPicorel Castaño, Rafael-
dc.contributor.authorYruela Guerrero, Inmaculada-
dc.date.accessioned2008-02-11T11:15:46Z-
dc.date.available2008-02-11T11:15:46Z-
dc.date.issued2006-06-
dc.identifier.citationPhysiologia Plantarum 127: 312-325 (2006)en_US
dc.identifier.issn0031-9317-
dc.identifier.urihttp://hdl.handle.net/10261/2909-
dc.description48 Pags.- 3 Tabls.- 6 Figs. The definitive version is available at: http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1399-3054-
dc.description.abstractThe influence of excess copper on soybean photosynthetic cell suspensions was investigated. The cell suspensions grew well in the presence of 5–20 µM CuSO4 and developed tolerance to even higher levels of CuSO4 (i.e. up to 50 µM), indicating that copper was not toxic to the cells at that high concentrations. Cu-adapted cell suspensions grew faster than the control in limiting light conditions and had higher content of chlorophyll per dry weight of cells. Copper was accumulated within the cells, and this event was accompanied by (1) increased oxygen evolution activity; (2) increased number of chloroplasts per cell, smaller chloroplasts, increased thylakoid stacking and grana size; (3) higher fluorescence emission of photosystem II antenna complexes and (4) stimulation of plastocyanin protein synthesis compared with untreated cells. Microanalysis of cross-sections revealed an increase of copper content in chloroplasts as well as vacuole, cytoplasm and cell wall in Cu-adapted cells. No antagonist interaction between copper and iron uptake took place in these cell suspensions. On the other hand, copper at subtoxic concentrations stimulated oxygen evolution activity in thylakoids from control cells, but this event did not take place in those from Cu-adapted ones. Furthermore, the loss of activity by copper inhibitory action at toxic concentrations was two-fold slower in thylakoids from Cu-adapted cells compared with the control ones. The data strongly indicate that copper plays a specific positive role on photosynthesis and stimulates the growth and the oxygen evolution activity in soybean cell suspensions.en_US
dc.description.sponsorshipM. Bernal is recipient of a predoctoral fellowship from Consejo Superior de Investigaciones Científicas (I3P Programme financed by the European Social Fund). This work was supported by the Aragón Government (Grant P015/2001) to I. Yruela, the Ministry of Science and Technology of Spain (BMC2002-00031) to R. Picorel, and it has been done within GC DGA 2002 programme of the Gobierno de Aragón.en_US
dc.format.extent816904 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoengen_US
dc.publisherBlackwell Publishingen_US
dc.rightsopenAccessen_US
dc.subjectCopper stressen_US
dc.subjectSoybeanen_US
dc.subjectChloroplasten_US
dc.subjectCell suspensionen_US
dc.titleExcess copper effect on growth, chloroplast ultrastructure, oxygen-evolution activity and chlorophyll fluorescence in Glycine max cell suspensionsen_US
dc.typeartículoen_US
dc.identifier.doihttp://dx.doi.org/10.1111/j.1399-3054.2006.00641.x-
dc.description.peerreviewedPeer revieweden_US
dc.relation.publisherversionhttp://dx.doi.org/10.1111/j.1399-3054.2006.00641.x-
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