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http://hdl.handle.net/10261/29036
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Buti, María | - |
dc.contributor.author | Jardi, R. | - |
dc.contributor.author | Rodríguez Frías, F. | - |
dc.contributor.author | Arranz, J. A. | - |
dc.contributor.author | Casacuberta, Josep M. | - |
dc.contributor.author | San Segundo, Blanca | - |
dc.date.accessioned | 2010-11-12T09:01:16Z | - |
dc.date.available | 2010-11-12T09:01:16Z | - |
dc.date.issued | 1991-01-01 | - |
dc.identifier.citation | European Journal of Clinical Chemistry and Clinical Biochemistry 29: 731-735 (1991) | es_ES |
dc.identifier.issn | 0939-4974 | - |
dc.identifier.uri | http://hdl.handle.net/10261/29036 | - |
dc.description | 6 pages, 1 figure, 1 table. | es_ES |
dc.description.abstract | The sensitivity and specificity of two non-radioactive spot hybridization assays for hepatitis B virus DNA (HBV-DNA) using biotin and digoxygenin-labelled DNA probes were investigated in parallel in 122 serum samples from patients with chronic hepatitis B and 50 controls. The results were compared with an isotopic technique using a 32P-labelled probe. | es_ES |
dc.description.abstract | HBV-DNA was detected in 56 (80%) out of 70 hepatitis B "e" antigen (HBeAg)-positive cases and in 4 (8%) out of 52 antibody to hepatitis B "e" antigen (anti-HBe)-positive cases using the digoxygenin or 32P-labelled probes. No false positives were found with either method. Using the biotin-labelled probe, 16% of sera gave discordant results, which were considered to be false positive. The time required for detection of serum HBVDNA was 2 hours for the non-radioactive probes and 16 hours for the isotopic probes. | es_ES |
dc.description.abstract | This study suggests that the digoxygenin-labelled probe for detection of HBV-DNA is the most rapid and sensitive method for routine diagnosis of viral replication in clinical laboratories. | es_ES |
dc.description.sponsorship | This work was supported in part by a grant from FISS 90-0938. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Walter de Gruyter | es_ES |
dc.rights | openAccess | es_ES |
dc.title | Digoxygenin-Labelled DNA-Probe: A Rapid Non-Radioactive Method for Hepatitis B Virus DNA Detection in Serum | es_ES |
dc.type | artículo | es_ES |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | http://edoc.hu-berlin.de/oa/degruyter/cclm.1991.29.11.731.pdf | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.fulltext | With Fulltext | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.openairetype | artículo | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
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Buti_Maria_et_al.pdf | 1,5 MB | Adobe PDF | Visualizar/Abrir |
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