Please use this identifier to cite or link to this item:
|Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL|
Digoxygenin-Labelled DNA-Probe: A Rapid Non-Radioactive Method for Hepatitis B Virus DNA Detection in Serum
|Authors:||Buti, María; Jardi, R.; Rodríguez Frías, F.; Arranz, J. A.; Casacuberta, Josep M. ; San Segundo, Blanca|
|Publisher:||Walter de Gruyter|
|Citation:||European Journal of Clinical Chemistry and Clinical Biochemistry 29: 731-735 (1991)|
|Abstract:||The sensitivity and specificity of two non-radioactive spot hybridization assays for hepatitis B virus DNA (HBV-DNA) using biotin and digoxygenin-labelled DNA probes were investigated in parallel in 122 serum samples from patients with chronic hepatitis B and 50 controls. The results were compared with an isotopic technique using a 32P-labelled probe.|
HBV-DNA was detected in 56 (80%) out of 70 hepatitis B "e" antigen (HBeAg)-positive cases and in 4 (8%) out of 52 antibody to hepatitis B "e" antigen (anti-HBe)-positive cases using the digoxygenin or 32P-labelled probes. No false positives were found with either method. Using the biotin-labelled probe, 16% of sera gave discordant results, which were considered to be false positive. The time required for detection of serum HBVDNA was 2 hours for the non-radioactive probes and 16 hours for the isotopic probes.
This study suggests that the digoxygenin-labelled probe for detection of HBV-DNA is the most rapid and sensitive method for routine diagnosis of viral replication in clinical laboratories.
|Description:||6 pages, 1 figure, 1 table.|
|Publisher version (URL):||http://edoc.hu-berlin.de/oa/degruyter/cclm.19184.108.40.2061.pdf|
|Appears in Collections:||(IQAC) Artículos|