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Digoxygenin-Labelled DNA-Probe: A Rapid Non-Radioactive Method for Hepatitis B Virus DNA Detection in Serum

AuthorsButi, María; Jardi, R.; Rodríguez Frías, F.; Arranz, J. A.; Casacuberta, Josep M. ; San Segundo, Blanca
Issue Date1-Jan-1991
PublisherWalter de Gruyter
CitationEuropean Journal of Clinical Chemistry and Clinical Biochemistry 29: 731-735 (1991)
AbstractThe sensitivity and specificity of two non-radioactive spot hybridization assays for hepatitis B virus DNA (HBV-DNA) using biotin and digoxygenin-labelled DNA probes were investigated in parallel in 122 serum samples from patients with chronic hepatitis B and 50 controls. The results were compared with an isotopic technique using a 32P-labelled probe.
HBV-DNA was detected in 56 (80%) out of 70 hepatitis B "e" antigen (HBeAg)-positive cases and in 4 (8%) out of 52 antibody to hepatitis B "e" antigen (anti-HBe)-positive cases using the digoxygenin or 32P-labelled probes. No false positives were found with either method. Using the biotin-labelled probe, 16% of sera gave discordant results, which were considered to be false positive. The time required for detection of serum HBVDNA was 2 hours for the non-radioactive probes and 16 hours for the isotopic probes.
This study suggests that the digoxygenin-labelled probe for detection of HBV-DNA is the most rapid and sensitive method for routine diagnosis of viral replication in clinical laboratories.
Description6 pages, 1 figure, 1 table.
Publisher version (URL)http://edoc.hu-berlin.de/oa/degruyter/cclm.1991.29.11.731.pdf
Appears in Collections:(IQAC) Artículos
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