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Título : Putative Porin of Bradyrhizobium sp. (Lupinus) Bacteroids Induced by Glyphosate
Autor : María, Nuria de; Guevara, Angeles; Serra, María Teresa ; García-Luque, Isabel ; González-Sama, Alfonso; García de Lacoba, Mario ; Felipe, Mª Rosario de; Fernández-Pascual, Mercedes
Fecha de publicación : 8-jun-2007
Editor: American Society for Microbiology
Citación : Applied and Environmental Microbiology: 73(16): 5075–5082 (2007)
Resumen: Application of glyphosate (N-[phosphonomethyl] glycine) to Bradyrhizobium sp. (Lupinus)-nodulated lupin plants caused modifications in the protein pattern of bacteroids. The most significant change was the presence of a 44-kDa polypeptide in bacteroids from plants treated with the higher doses of glyphosate employed (5 and 10 mM). The polypeptide has been characterized by the amino acid sequencing of its N terminus and the isolation and nucleic acid sequencing of its encoding gene. It is putatively encoded by a single gene, and the protein has been identified as a putative porin. Protein modeling revealed the existence of several domains sharing similarity to different porins, such as a transmembrane beta-barrel. The protein has been designated BLpp, for Bradyrhizobium sp. (Lupinus) putative porin, and would be the first porin described in Bradyrhizobium sp. (Lupinus). In addition, a putative conserved domain of porins has been identified which consists of 87 amino acids, located in the BLpp sequence 30 amino acids downstream of the N-terminal region. In bacteroids, mRNA of the BLpp gene shows a basal constitutive expression that increases under glyphosate treatment, and the expression of the gene is seemingly regulated at the transcriptional level. By contrast, in free-living bacteria glyphosate treatment leads to an inhibition of BLpp mRNA accumulation, indicating a different effect of glyphosate on BLpp gene expression in bacteroids and free-living bacteria. The possible role of BLpp in a metabolite interchange between Bradyrhizobium and lupin is discussed.
Versión del editor: http://dx.doi.org/10.1128/AEM.00392-07
URI : http://hdl.handle.net/10261/2841
DOI: 10.1128/AEM.00392-07
ISSN: 0099-2240
E-ISSN: 1098-5336
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