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Título: | Telomeric C-circles localize at nuclear pore complexes in Saccharomyces cerevisiae |
Autor: | Aguilera, Paula CSIC ORCID; Dubarry, Marion; Hardy, Julien; Lisby, Michael; Simon, Marie-Noelle; Geli, Vicent | Palabras clave: | Alternative lengthening of telomeres C-circles Recombination senescence Telomeres |
Fecha de publicación: | 15-mar-2022 | Editor: | EMBO Press | Citación: | EMBO Journal 41(6): e108736 (2022) | Resumen: | As in human cells, yeast telomeres can be maintained in cells lacking telomerase activity by recombination-based mechanisms known as ALT (Alternative Lengthening of Telomeres). A hallmark of ALT human cancer cells are extrachromosomal telomeric DNA elements called C-circles, whose origin and function have remained unclear. Here, we show that extrachromosomal telomeric C-circles in yeast can be detected shortly after senescence crisis and concomitantly with the production of survivors arising from “type II” recombination events. We uncover that C-circles bind to the nuclear pore complex (NPC) and to the SAGA-TREX2 complex, similar to other non-centromeric episomal DNA. Disrupting the integrity of the SAGA/TREX2 complex affects both C-circle binding to NPCs and type II telomere recombination, suggesting that NPC tethering of C-circles facilitates formation and/or propagation of the long telomere repeats characteristic of type II survivors. Furthermore, we find that disruption of the nuclear diffusion barrier impairs type II recombination. These results support a model in which concentration of C-circles at NPCs benefits type II telomere recombination, highlighting the importance of spatial coordination in ALT-type mechanisms of telomere maintenance. | Versión del editor: | http://dx.doi.org/10.15252/embj.2021108736 | URI: | http://hdl.handle.net/10261/283045 | DOI: | 10.15252/embj.2021108736 | Identificadores: | doi: 10.15252/embj.2021108736 e-issn: 1460-2075 issn: 0261-4189 |
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